PMID- 19485975
OWN - NLM
STAT- MEDLINE
DCOM- 20091116
LR  - 20211020
IS  - 1530-0277 (Electronic)
IS  - 0145-6008 (Print)
IS  - 0145-6008 (Linking)
VI  - 33
IP  - 9
DP  - 2009 Sep
TI  - Alcohol exposure impairs myeloid dendritic cell function in rhesus macaques.
PG  - 1524-31
LID - 10.1111/j.1530-0277.2009.00980.x [doi]
AB  - BACKGROUND: Alcohol intoxication suppresses both the innate and adaptive 
      immunities. Dendritic cells (DCs) are the major cell type bridging the innate and 
      acquired immune responses. At the present time, the effects of alcohol on DC 
      development in hematopoietic tissues and the functional activities of DCs are 
      incompletely elucidated. This study investigated the impact of chronic alcohol 
      exposure on the alteration of hematopoietic precursor cell and DC populations in 
      the bone marrow and peripheral blood of rhesus macaques. METHODS: Rhesus macaques 
      were administered alcohol or isocaloric sucrose daily for a period of 3 months 
      through surgically implanted gastric catheters. Peripheral blood mononuclear 
      cells (PBMCs) and bone marrow cells (BMCs) were isolated for flow cytometric 
      analysis after 3 months. Monocytes were cultured with human IL-4 (10 ng/ml) and 
      GM-CSF (50 ng/ml) in the absence and presence of alcohol (50 mM). On day 6 of the 
      culture, a cocktail of stimulants including IL-1beta (18 ng), IL-6 (1800 U), 
      TNF-alpha (18 ng), and PGE(2) (1.8 microg) were added to the designated wells for 
      transformation of immature dendritic cells (iDCs) to mature myeloid DCs. The 
      cells were analyzed on day 8 by flow cytometry for expression of DC costimulatory 
      molecule expression. RESULTS: EtOH-treated animals had significantly lower 
      numbers of myeloid DCs (lineage-HLA-DR+CD11c+CD123-) in both the PBMCs and BMCs 
      compared to controls (5,654 +/- 1,273/10(6) vs. 2,353 +/- 660/10(6) PBMCs and 503 
      +/- 34 vs. 195 +/- 44/10(6) BMCs). Under culture conditions, the number of 
      lineage-HLA-DR+CD83+ cells was low in control wells (0.38 +/- 0.08%). Alcohol 
      inhibited the increase in the number of lineage-HLA-DR+CD83+ cells in iDC wells 
      (2.30 +/- 0.79% vs. 5.73 +/- 1.40%). Alcohol also inhibited the increase in the 
      number of lineage-HLA-DR+CD83+ cells in mature DC wells (1.23 +/- 0.15% vs. 4.13 
      +/- 0.62%). CONCLUSIONS: Chronic EtOH decreases the bone marrow and circulating 
      pools of myeloid DCs. Additionally, EtOH suppresses costimulatory molecule CD83 
      expression during DC transformation, which may attenuate the ability of DCs to 
      initiate T-cell expansion.
FAU - Siggins, Robert W
AU  - Siggins RW
AD  - Alcohol Research Center, Louisiana State University Health Sciences Center, New 
      Orleans, LA 70112-1393, USA.
FAU - Bagby, Gregory J
AU  - Bagby GJ
FAU - Molina, Patricia
AU  - Molina P
FAU - Dufour, Jason
AU  - Dufour J
FAU - Nelson, Steve
AU  - Nelson S
FAU - Zhang, Ping
AU  - Zhang P
LA  - eng
GR  - P60 AA009803/AA/NIAAA NIH HHS/United States
GR  - AA07577/AA/NIAAA NIH HHS/United States
GR  - R01 AA013543/AA/NIAAA NIH HHS/United States
GR  - R01 AA013543-04S1/AA/NIAAA NIH HHS/United States
GR  - R01 AA011290/AA/NIAAA NIH HHS/United States
GR  - P60 AA009803-16S1/AA/NIAAA NIH HHS/United States
GR  - AA11290/AA/NIAAA NIH HHS/United States
GR  - R01 AA011290-15/AA/NIAAA NIH HHS/United States
GR  - T32 AA007577/AA/NIAAA NIH HHS/United States
GR  - T32 AA007577-09/AA/NIAAA NIH HHS/United States
GR  - R37 AA011290/AA/NIAAA NIH HHS/United States
GR  - AA09803/AA/NIAAA NIH HHS/United States
GR  - P50 AA009803/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090526
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 0 (Central Nervous System Depressants)
RN  - 0 (Membrane Proteins)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*drug effects
MH  - Cells, Cultured
MH  - Central Nervous System Depressants/*toxicity
MH  - Dendritic Cells/*drug effects
MH  - Ethanol/*toxicity
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/*drug effects
MH  - Macaca mulatta
MH  - Membrane Proteins/biosynthesis
MH  - Monocytes/drug effects
PMC - PMC2948236
MID - NIHMS235407
EDAT- 2009/06/03 09:00
MHDA- 2009/11/17 06:00
PMCR- 2010/10/01
CRDT- 2009/06/03 09:00
PHST- 2009/06/03 09:00 [entrez]
PHST- 2009/06/03 09:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - ACER980 [pii]
AID - 10.1111/j.1530-0277.2009.00980.x [doi]
PST - ppublish
SO  - Alcohol Clin Exp Res. 2009 Sep;33(9):1524-31. doi: 
      10.1111/j.1530-0277.2009.00980.x. Epub 2009 May 26.