PMID- 19486156
OWN - NLM
STAT- MEDLINE
DCOM- 20090918
LR  - 20240109
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 69
IP  - 1
DP  - 2009 Jul
TI  - In vitro antagonists of Rhizoctonia solani tested on lettuce: rhizosphere 
      competence, biocontrol efficiency and rhizosphere microbial community response.
PG  - 62-74
LID - 10.1111/j.1574-6941.2009.00685.x [doi]
AB  - The rhizosphere competence of 15 in vitro antagonists of Rhizoctonia solani was 
      determined 4 weeks after sowing inoculated lettuce seeds into nonsterile soil. 
      Based on the colonization ability determined by selective plating, eight strains 
      were selected for growth chamber experiments to determine their efficacy in 
      controlling bottom rot caused by R. solani on lettuce. Although in the first 
      experiment all antagonists colonized the rhizosphere of lettuce with CFU counts 
      above 2 x 10(6) g(-1) of root fresh weight, only four isolates significantly 
      reduced disease severity. In subsequent experiments involving these four 
      antagonists, only Pseudomonas jessenii RU47 showed effective and consistent 
      disease suppression. Plate counts and denaturing gradient gel electrophoresis 
      (DGGE) fingerprints of Pseudomonas-specific gacA genes amplified from total 
      community DNA confirmed that RU47 established as the dominant Pseudomonas 
      population in the rhizosphere of inoculated lettuce plants. Furthermore, the DGGE 
      fingerprint revealed that R. solani AG1-IB inoculation severely affected the 
      bacterial and fungal community structure in the rhizosphere of lettuce and that 
      these effects were much less pronounced in the presence of RU47. Although the 
      exact mechanism of antagonistic activity and the ecology of RU47 remain to be 
      further explored, our results suggest that RU47 is a promising agent to control 
      bottom rot of lettuce.
FAU - Adesina, Modupe F
AU  - Adesina MF
AD  - Julius Kuhn-Institut - Federal Research Centre for Cultivated Plants (JKI), 
      D-38104 Braunschweig, Germany.
FAU - Grosch, Rita
AU  - Grosch R
FAU - Lembke, Antje
AU  - Lembke A
FAU - Vatchev, Tzenko D
AU  - Vatchev TD
FAU - Smalla, Kornelia
AU  - Smalla K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090522
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - *Antibiosis
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/genetics
MH  - Lactuca/*microbiology
MH  - Plant Diseases/*microbiology
MH  - Plant Roots/microbiology
MH  - Pseudomonas/genetics/growth & development
MH  - RNA, Ribosomal, 16S/genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - Rhizoctonia/*growth & development
MH  - *Soil Microbiology
EDAT- 2009/06/03 09:00
MHDA- 2009/09/19 06:00
CRDT- 2009/06/03 09:00
PHST- 2009/06/03 09:00 [entrez]
PHST- 2009/06/03 09:00 [pubmed]
PHST- 2009/09/19 06:00 [medline]
AID - FEM685 [pii]
AID - 10.1111/j.1574-6941.2009.00685.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2009 Jul;69(1):62-74. doi: 10.1111/j.1574-6941.2009.00685.x. 
      Epub 2009 May 22.