PMID- 19490633
OWN - NLM
STAT- MEDLINE
DCOM- 20100322
LR  - 20211020
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 11
IP  - 3
DP  - 2009
TI  - Nerve growth factor and receptor expression in rheumatoid arthritis and 
      spondyloarthritis.
PG  - R82
LID - 10.1186/ar2716 [doi]
AB  - INTRODUCTION: We previously described the presence of nerve growth factor 
      receptors in the inflamed synovial compartment. Here we investigated the presence 
      of the corresponding nerve growth factors, with special focus on nerve growth 
      factor (NGF). METHODS: mRNA expression levels of four ligands (NGF, brain derived 
      growth factor (BDNF), neurotrophin (NT)-3, NT-4) and their four corresponding 
      receptors (tyrosine kinase (trk) A, trkB, trkC, NGFRp75) were determined in the 
      synovial fluid (SF) cells of 9 patients with rheumatoid arthritis (RA) and 16 
      with spondyloarthritis (SpA) and compared with 7 osteoarthritis (OA) patients. 
      NGF was also determined in synovial tissue (ST) biopsies of 10 RA and 10 SpA 
      patients. The production of NGF by monocytes and lymphocytes was assessed by flow 
      cytometry of SF cells, synovial tissue derived fibroblast-like synoviocytes (FLS) 
      were assessed by ELISA on culture supernatant. RESULTS: SF cell analysis revealed 
      a clear BDNF and NGF mRNA expression, with significantly higher NGF expression in 
      RA and SpA patients than in the OA group. NGF expression was higher in ST samples 
      of RA as compared to SpA. Using intracellular FACS analysis, we could demonstrate 
      the presence of the NGF protein in the two inflammatory arthritis groups on both 
      CD3+ T lymphocytes and CD14+ cells, i.e. monocytes/macrophages, whereas cultured 
      FLS did not produce NGF in vitro. CONCLUSIONS: Neurotrophins and especially NGF 
      are expressed in the synovial fluid and tissue of patients with peripheral 
      synovitis. The presence of neurotrophins as well as their receptors, in 
      particular the NGF/trkA-p75 axis in peripheral synovitis warrants further 
      functional investigation of their active involvement in chronic inflammatory 
      arthritis.
FAU - Barthel, Christian
AU  - Barthel C
AD  - Clinic for Immunology and Rheumatology, Hannover Medical School (MHH), 
      Carl-Neuberg-Strasse 1, Hannover 30625, Germany. sanoa@t-online.de
FAU - Yeremenko, Nataliya
AU  - Yeremenko N
FAU - Jacobs, Roland
AU  - Jacobs R
FAU - Schmidt, Reinhold E
AU  - Schmidt RE
FAU - Bernateck, Michael
AU  - Bernateck M
FAU - Zeidler, Henning
AU  - Zeidler H
FAU - Tak, Paul-Peter
AU  - Tak PP
FAU - Baeten, Dominique
AU  - Baeten D
FAU - Rihl, Markus
AU  - Rihl M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090602
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Arthritis, Rheumatoid/genetics/*metabolism
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nerve Growth Factor/*biosynthesis/genetics
MH  - Receptors, Nerve Growth Factor/*biosynthesis/genetics
MH  - Spondylarthritis/genetics/*metabolism
MH  - Synovial Fluid/metabolism
MH  - Young Adult
PMC - PMC2714133
EDAT- 2009/06/06 09:00
MHDA- 2010/03/23 06:00
PMCR- 2009/06/02
CRDT- 2009/06/04 09:00
PHST- 2008/11/12 00:00 [received]
PHST- 2009/05/11 00:00 [revised]
PHST- 2009/06/02 00:00 [accepted]
PHST- 2009/06/04 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2010/03/23 06:00 [medline]
PHST- 2009/06/02 00:00 [pmc-release]
AID - ar2716 [pii]
AID - 10.1186/ar2716 [doi]
PST - ppublish
SO  - Arthritis Res Ther. 2009;11(3):R82. doi: 10.1186/ar2716. Epub 2009 Jun 2.