PMID- 19490757
OWN - NLM
STAT- MEDLINE
DCOM- 20090803
LR  - 20171116
IS  - 1607-8454 (Electronic)
IS  - 1024-5332 (Linking)
VI  - 14
IP  - 3
DP  - 2009 Jun
TI  - Correlation between flow cytometric identification of CD33-positive cells and 
      morphological evaluation of myeloblasts in bone marrow of patients with acute 
      myeloblastic leukemia.
PG  - 133-8
LID - 10.1179/102453309X402269 [doi]
AB  - Determination of the percentage of myeloblasts in bone marrow is important for 
      the evaluation of acute myeloblastic leukemia (AML) and related disorders. Using 
      flow cytometry with a CD45-blast gate (FCM/CD45), 226 bone marrow aspiration 
      samples serially collected from 71 patients with de novo AML were analyzed. Bone 
      marrow smears were evaluated independently by pathologists who did not know the 
      corresponding flow cytometric data in advance. Patients received remission 
      induction followed by consolidation. The CD33+ cell percentages evaluated by 
      FCM/CD45 were strongly correlated to the myeloblast percentages determined by 
      microscopic examination (r=0.8360, p<0.001). When only samples containing 
      leukemic cells demonstrated by chromosomal or fluorescence in situ hybridization 
      (FISH) analysis after induction were evaluated, positive correlations were found 
      between CD33+ cell percentages determined by FCM/CD45 and myeloblast percentages 
      determined by morphology (r=0.672, p<0.001). The identification of CD33+ cells by 
      FCM/CD45 is useful for the evaluation of bone marrow myeloblasts in AML.
FAU - Oka, Satoko
AU  - Oka S
AD  - Division of Hematology, Department of Medicine, Jichi Medical University, 
      Shimotsuke, Tochigi, Japan.
FAU - Muroi, Kazuo
AU  - Muroi K
FAU - Matsuyama, Tomohiro
AU  - Matsuyama T
FAU - Sato, Kazuya
AU  - Sato K
FAU - Ueda, Masuzu
AU  - Ueda M
FAU - Toshima, Masaki
AU  - Toshima M
FAU - Suzuki, Takahiro
AU  - Suzuki T
FAU - Ozaki, Katsutoshi
AU  - Ozaki K
FAU - Mori, Masaki
AU  - Mori M
FAU - Takubo, Takayuki
AU  - Takubo T
FAU - Nagai, Tadashi
AU  - Nagai T
FAU - Hanafusa, Toshiaki
AU  - Hanafusa T
FAU - Ozawa, Keiya
AU  - Ozawa K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD33 protein, human)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 3)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/*immunology
MH  - Antigens, Differentiation, Myelomonocytic/*immunology
MH  - Bone Marrow/immunology/pathology
MH  - Bone Marrow Cells/*cytology/immunology
MH  - Flow Cytometry/*methods
MH  - Granulocyte Precursor Cells/*cytology/immunology
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*diagnosis/drug therapy/immunology
MH  - Leukocyte Common Antigens/*immunology
MH  - Middle Aged
MH  - Neoplasm, Residual
MH  - Phenotype
MH  - Sialic Acid Binding Ig-like Lectin 3
EDAT- 2009/06/06 09:00
MHDA- 2009/08/04 09:00
CRDT- 2009/06/04 09:00
PHST- 2009/06/04 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/08/04 09:00 [medline]
AID - 10.1179/102453309X402269 [doi]
PST - ppublish
SO  - Hematology. 2009 Jun;14(3):133-8. doi: 10.1179/102453309X402269.