PMID- 19490982
OWN - NLM
STAT- MEDLINE
DCOM- 20090716
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 159
IP  - 2
DP  - 2009 Aug
TI  - Use of the Roche LightCycler 480 system in a routine laboratory setting for 
      molecular diagnosis of opportunistic viral infections: Evaluation on whole blood 
      specimens and proficiency panels.
PG  - 291-4
LID - 10.1016/j.jviromet.2009.03.027 [doi]
AB  - The Roche LightCycler 480 (LC480) system was evaluated for quantitative molecular 
      diagnosis of opportunistic viral infections caused by human cytomegalovirus 
      (HCMV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6) and BK virus (BKV), 
      in comparison with "in-house" real-time PCR assays. A total of 253 whole blood 
      specimens obtained from transplant recipients were tested. Both the "in-house" 
      and the LC480 methods were highly correlated (Spearman correlation coefficient 
      Rho>or=0.85; p<0.0001) with an excellent overall qualitative agreement (90.5%) 
      and no significant quantitative difference between both techniques for the four 
      viruses tested. The accuracy of the LC480 protocols were confirmed further by the 
      results obtained with the 44 samples from the Quality Control for Molecular 
      Diagnosis (QCMD) 2008 proficiency panel. The LC480 system constitutes a suitable 
      and versatile real-time PCR platform in a routine laboratory setting for the 
      diagnosis and monitoring of opportunistic viral infections in transplant 
      recipients, by measuring HCMV, EBV, HHV-6, and BKV loads in whole blood samples.
FAU - Deback, Claire
AU  - Deback C
AD  - UPMC Univ Paris 06, ER1 DETIV, F-75013 Paris, France.
FAU - Geli, Julienne
AU  - Geli J
FAU - Ait-Arkoub, Zaina
AU  - Ait-Arkoub Z
FAU - Angleraud, Francis
AU  - Angleraud F
FAU - Gautheret-Dejean, Agnes
AU  - Gautheret-Dejean A
FAU - Agut, Henri
AU  - Agut H
FAU - Boutolleau, David
AU  - Boutolleau D
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20090409
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - BK Virus/isolation & purification
MH  - Blood/*virology
MH  - Cytomegalovirus/isolation & purification
MH  - Herpesvirus 4, Human/isolation & purification
MH  - Herpesvirus 6, Human/isolation & purification
MH  - Humans
MH  - Molecular Diagnostic Techniques/*methods
MH  - Polymerase Chain Reaction/*methods
MH  - Virus Diseases/*diagnosis
MH  - Viruses/*isolation & purification
EDAT- 2009/06/06 09:00
MHDA- 2009/07/17 09:00
CRDT- 2009/06/04 09:00
PHST- 2009/02/02 00:00 [received]
PHST- 2009/03/24 00:00 [revised]
PHST- 2009/03/31 00:00 [accepted]
PHST- 2009/06/04 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/07/17 09:00 [medline]
AID - S0166-0934(09)00162-1 [pii]
AID - 10.1016/j.jviromet.2009.03.027 [doi]
PST - ppublish
SO  - J Virol Methods. 2009 Aug;159(2):291-4. doi: 10.1016/j.jviromet.2009.03.027. Epub 
      2009 Apr 9.