PMID- 19492919
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20161125
IS  - 1619-3997 (Electronic)
IS  - 0300-5577 (Linking)
VI  - 37
IP  - 5
DP  - 2009
TI  - Nicotine affects the expression of brain-derived neurotrophic factor mRNA and 
      protein in the hippocampus of hypoxic newborn piglets.
PG  - 553-60
LID - 10.1515/JPM.2009.081 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is highly expressed in the developing 
      brain. It has anti-apoptotic abilities, and protects the neonatal brain. In 
      experimental settings in adult animals, pre-treatment with nicotine has shown 
      increased BDNF levels, indicating a possible contribution to nicotine's 
      anti-apoptotic effect. Apoptosis contributes to the development of brain damage 
      in perinatal asphyxia. We examined the effects of nicotine on apoptosis-inducing 
      factor (AIF), caspase-3 and BDNF in the hippocampus of a neonatal piglet model of 
      global hypoxia. Forty-one anesthetized newborn piglets were randomized to one of 
      four groups receiving different infusions after hypoxia (1) nicotine 130 
      microg/kg/h, 2) 260 microg/kg/h, 3) adrenaline, and 4) saline, all 2.6 mL/kg/h. 
      Four hours after hypoxia they were euthanized. The left hemisphere/hippocampus 
      was examined by histopathology and immunohistochemistry; the right hippocampus 
      was analyzed using real time PCR. There was a significantly higher expression of 
      BDNF mRNA and protein in the animals treated with nicotine 130 microg/kg/h vs. 
      the saline treated group (mRNA P=0.038; protein P=0.009). There were no 
      differences regarding AIF or caspase-3. We conclude that nicotine (130 
      microg/kg/h), infused over 1 h after global hypoxia in neonatal piglets, 
      increases levels of both BDNF mRNA and protein in the hippocampus. This might 
      imply neuroprotective effects of nicotine in asphyxiated neonates.
FAU - Andresen, Jannicke Hanne
AU  - Andresen JH
AD  - Department of Pediatric Research, Institute of Surgical Research, Medical 
      Faculty, University of Oslo, Rikshospitalet University Hospital, Oslo, Norway. 
      jannickea@adhb.govt.nz
FAU - Loberg, Else Marit
AU  - Loberg EM
FAU - Wright, Marianne
AU  - Wright M
FAU - Goverud, Ingeborg Lostegaard
AU  - Goverud IL
FAU - Stray-Pedersen, Babill
AU  - Stray-Pedersen B
FAU - Saugstad, Ola Didrik
AU  - Saugstad OD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Perinat Med
JT  - Journal of perinatal medicine
JID - 0361031
RN  - 0 (Apoptosis Inducing Factor)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (RNA, Messenger)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Apoptosis Inducing Factor/genetics
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/*genetics/*metabolism
MH  - Caspase 3/genetics
MH  - DNA Primers/genetics
MH  - Disease Models, Animal
MH  - Gene Expression/drug effects
MH  - Hippocampus/*drug effects/*metabolism/pathology
MH  - Hypoxia/*genetics/*metabolism/pathology
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neuroprotective Agents/pharmacology
MH  - Nicotine/*pharmacology
MH  - RNA, Messenger/*genetics/*metabolism
MH  - Swine
MH  - Tissue Distribution
EDAT- 2009/06/06 09:00
MHDA- 2010/02/03 06:00
CRDT- 2009/06/05 09:00
PHST- 2009/06/05 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
AID - 10.1515/JPM.2009.081 [doi]
PST - ppublish
SO  - J Perinat Med. 2009;37(5):553-60. doi: 10.1515/JPM.2009.081.