PMID- 19493662 OWN - NLM STAT- MEDLINE DCOM- 20100813 LR - 20131121 IS - 1618-1433 (Electronic) IS - 0940-2993 (Linking) VI - 62 IP - 3 DP - 2010 May TI - Evaluation of the hepatotoxic and hepatoprotective effect of Rwandese herbal drugs on in vivo (guinea pigs barbiturate-induced sleeping time) and in vitro (rat precision-cut liver slices, PCLS) models. PG - 289-99 LID - 10.1016/j.etp.2009.04.005 [doi] AB - Precision-cut liver slices (PCLS) preserve the tissular organization of the organ and represent an in vitro model closer to in vivo conditions than hepatocytes cultures. As this may be an interesting tool not only for the investigation of hepatotoxic and protective effects but also for bioguided fractionations schemes, the usefulness of PCLS was compared with an in vivo test of liver function. Crude extracts derived from five herbs used in Rwanda for hepatoprotective activity were tested on CCl(4)-treated guinea pigs by the method of barbiturate-induced sleep modification. Aqueous extracts of Ocimum lamiifolium, Crassocephalum vitellinum, Guizotia scabra and Vernonia lasiopus leaves allowed animals to recover barbiturate sleep duration in proportions of 88%, 78%, 61% and 34%, respectively and Microglossa pyrifolia was found inactive. Dried methanolic extracts of the 5 plants were then tested in vitro on rat PCLS for protection against acetaminophen-induced hepatotoxicity. In this model, G. scabra, M. pyrifolia and V. lasiopus were found hepatotoxic by themselves and unable to prevent acetaminophen toxicity. The most active extract, obtained from O. lamiifolium, was subjected to bioassay-guided fractionation by chromatography on Si-C(18) to yield two quite active fractions. From a single animal, at least 50 PCLS explants can be prepared, which allows testing large amounts of samples, strengthening ethnopharmacological data on hepatoprotective medicinal plants and investigating hepatotoxic effects. FAU - Mukazayire, Marie-Jeanne AU - Mukazayire MJ AD - Laboratoire de Pharmacognosie, de Bromatologie et de Nutrition Humaine, Institut de Pharmacie, Universite Libre de Bruxelles (ULB), Campus de la Plaine-CP 205/9, Bd du Triomphe, B-1050 Bruxelles, Belgique. mmukazay@ulb.ac.be FAU - Allaeys, Veronique AU - Allaeys V FAU - Buc Calderon, Pedro AU - Buc Calderon P FAU - Stevigny, Caroline AU - Stevigny C FAU - Bigendako, Marie-Josee AU - Bigendako MJ FAU - Duez, Pierre AU - Duez P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090603 PL - Germany TA - Exp Toxicol Pathol JT - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie JID - 9208920 RN - 0 (Analgesics, Non-Narcotic) RN - 0 (Barbiturates) RN - 0 (Hypnotics and Sedatives) RN - 0 (Plant Extracts) RN - 362O9ITL9D (Acetaminophen) SB - IM MH - Acetaminophen/toxicity MH - Analgesics, Non-Narcotic/toxicity MH - Animals MH - Barbiturates/pharmacology MH - Disease Models, Animal MH - Female MH - Guinea Pigs MH - Hypnotics and Sedatives/pharmacology MH - Liver/*drug effects MH - Male MH - Ocimum/chemistry MH - Organ Culture Techniques/*methods MH - Phytotherapy/*methods MH - Plant Extracts/chemistry/*pharmacology MH - Plant Leaves/chemistry MH - Plants, Medicinal/*chemistry MH - Rats MH - Rwanda MH - Sleep/*drug effects EDAT- 2009/06/06 09:00 MHDA- 2010/08/14 06:00 CRDT- 2009/06/05 09:00 PHST- 2008/07/14 00:00 [received] PHST- 2009/01/30 00:00 [revised] PHST- 2009/04/22 00:00 [accepted] PHST- 2009/06/05 09:00 [entrez] PHST- 2009/06/06 09:00 [pubmed] PHST- 2010/08/14 06:00 [medline] AID - S0940-2993(09)00171-7 [pii] AID - 10.1016/j.etp.2009.04.005 [doi] PST - ppublish SO - Exp Toxicol Pathol. 2010 May;62(3):289-99. doi: 10.1016/j.etp.2009.04.005. Epub 2009 Jun 3.