PMID- 19494503 OWN - NLM STAT- MEDLINE DCOM- 20090720 LR - 20141120 IS - 1423-0097 (Electronic) IS - 1018-2438 (Linking) VI - 149 Suppl 1 DP - 2009 TI - Amphiregulin production by human eosinophils. PG - 39-44 LID - 10.1159/000210652 [doi] AB - BACKGROUND: Amphiregulin (AREG) plays critical roles in mammary gland development, immune responses against nematode infection, and mucous production in the lung. Since remarkable eosinophil infiltration has been shown at all of these tissue sites, we examined AREG production by human eosinophils in vitro. METHODS: Using Ficoll and antibody-coated immunomagnetic beads, eosinophils and other blood cells were purified from peripheral blood samples obtained from normal or mild allergic patients. Eosinophils were cultured in the presence of 10 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF), 10 ng/ml of IL-5, 100 ng/ml of IFN-gamma or immobilized secretory IgA. Total mRNA was extracted after 6 h of culture, and mRNA expression was measured using a microarray and RT-PCR. The AREG concentration in the supernatant and cell lysate after 48 h of culture was measured using ELISA. RESULTS: AREG mRNA was constitutively expressed in fresh eosinophils, and the expression level in the eosinophils was higher than that in other types of blood cells. AREG mRNA increased significantly upon stimulation with GM-CSF and IL-5 but not with IFN-gamma. AREG mRNA expression in GM-CSF-stimulated eosinophils was enhanced by dexamethasone. Fresh eosinophils did not contain AREG protein. AREG was significantly released into the supernatant when the eosinophils were stimulated with GM-CSF, but not with IFN-gamma or immobilized secretory IgA. CONCLUSION: Our data suggested that eosinophils produce and release AREG, and participate in some physiological and pathological conditions in vivo. CI - Copyright 2009 S. Karger AG, Basel. FAU - Matsumoto, Kenji AU - Matsumoto K AD - Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan. FAU - Fukuda, Shuhei AU - Fukuda S FAU - Nakamura, Yuri AU - Nakamura Y FAU - Saito, Hirohisa AU - Saito H LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090603 PL - Switzerland TA - Int Arch Allergy Immunol JT - International archives of allergy and immunology JID - 9211652 RN - 0 (AREG protein, human) RN - 0 (Amphiregulin) RN - 0 (EGF Family of Proteins) RN - 0 (Glycoproteins) RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (Interleukin-5) RN - 0 (RNA, Messenger) RN - 7S5I7G3JQL (Dexamethasone) RN - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor) SB - IM MH - Amphiregulin MH - Cells, Cultured MH - Dexamethasone/pharmacology MH - EGF Family of Proteins MH - Enzyme-Linked Immunosorbent Assay MH - Eosinophils/drug effects/*metabolism MH - Glycoproteins/analysis/*biosynthesis/genetics MH - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology MH - Humans MH - Intercellular Signaling Peptides and Proteins/analysis/*biosynthesis/genetics MH - Interleukin-5/pharmacology MH - Polymerase Chain Reaction MH - Protein Array Analysis MH - RNA, Messenger/analysis MH - Up-Regulation EDAT- 2009/06/12 09:00 MHDA- 2009/07/21 09:00 CRDT- 2009/06/05 09:00 PHST- 2009/06/05 09:00 [entrez] PHST- 2009/06/12 09:00 [pubmed] PHST- 2009/07/21 09:00 [medline] AID - 000210652 [pii] AID - 10.1159/000210652 [doi] PST - ppublish SO - Int Arch Allergy Immunol. 2009;149 Suppl 1:39-44. doi: 10.1159/000210652. Epub 2009 Jun 3.