PMID- 19494521
OWN - NLM
STAT- MEDLINE
DCOM- 20091229
LR  - 20111117
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 150
IP  - 3
DP  - 2009
TI  - Differential protein expression by dendritic cells from atopic and non-atopic 
      individuals after stimulation by the major house dust mite allergen Der p 1.
PG  - 237-51
LID - 10.1159/000222676 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are sentinels of the immune system and are 
      known to play a key role in allergic responses. However, it is not clear how DCs 
      that have been exposed to an allergen support Th2 type immune responses. It is 
      possible that DCs from atopic individuals are inherently programmed to support 
      allergic disease, or it is the exposure of dendritic cells to allergens that is 
      key to the development of allergic sensitisation. METHODS: We used 2D gel 
      electrophoresis and MALDI mass spectrometry to compare the proteome of DCs from 
      atopic and non-atopic individuals in both the resting state and after stimulation 
      with the major house dust mite allergen Der p 1. RESULTS: Our data show that 
      unstimulated DCs from atopic and non-atopic individuals are very similar at the 
      whole cell proteome level, showing few differentially expressed proteins. 
      However, upon stimulation with Der p 1, a number of additional proteins are 
      differentially expressed, and of these several were of potential relevance to Th2 
      cell differentiation and the allergic response, including GTP-binding regulatory 
      protein Gi alpha-2, frabin and cathepsin D. CONCLUSION: Whilst there are inherent 
      differences between DCs from atopic and non-atopic individuals, it seems that 
      exposure to allergen plays a key role in differential expression of proteins by 
      these key immune cells. Further studies should now focus on establishing the 
      biological relevance of these proteins as biomarkers in house dust mite allergy 
      and their role in allergen induced Th2 cell differentiation.
CI  - Copyright 2009 S. Karger AG, Basel.
FAU - Horlock, C
AU  - Horlock C
AD  - Institute of Infection, Immunity and Inflammation, School of Molecular Medical 
      Sciences and Biomedical Research Unit, University of Nottingham, Nottingham, UK.
FAU - Shakib, F
AU  - Shakib F
FAU - Jones, N S
AU  - Jones NS
FAU - Sewell, H F
AU  - Sewell HF
FAU - Ghaemmaghami, A M
AU  - Ghaemmaghami AM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090604
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (FGD4 protein, human)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Proteome)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
RN  - EC 3.4.23.5 (Cathepsin D)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gi-Go)
SB  - IM
MH  - Adult
MH  - Allergens/*immunology
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Arthropod Proteins
MH  - Cathepsin D/genetics/immunology/metabolism
MH  - Cells, Cultured
MH  - Cysteine Endopeptidases
MH  - Dendritic Cells/immunology/*metabolism/pathology
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Female
MH  - GTP-Binding Protein alpha Subunits, Gi-Go/genetics/immunology/metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - Hypersensitivity, Immediate/genetics/*immunology/*metabolism/pathology
MH  - Male
MH  - Microfilament Proteins/genetics/immunology/metabolism
MH  - Middle Aged
MH  - Proteome
MH  - Pyroglyphidae/immunology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Th2 Cells/immunology
EDAT- 2009/06/06 09:00
MHDA- 2009/12/30 06:00
CRDT- 2009/06/05 09:00
PHST- 2008/09/30 00:00 [received]
PHST- 2009/02/16 00:00 [accepted]
PHST- 2009/06/05 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/12/30 06:00 [medline]
AID - 000222676 [pii]
AID - 10.1159/000222676 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2009;150(3):237-51. doi: 10.1159/000222676. Epub 2009 
      Jun 4.