PMID- 19495460
OWN - NLM
STAT- MEDLINE
DCOM- 20090729
LR  - 20090604
IS  - 1473-0197 (Print)
IS  - 1473-0189 (Linking)
VI  - 9
IP  - 12
DP  - 2009 Jun 21
TI  - Microfabricated curtains for controlled cell seeding in high throughput 
      microfluidic systems.
PG  - 1756-62
LID - 10.1039/b819622b [doi]
AB  - A microfabricated cell curtain is presented that facilitates cellular assays. The 
      cell curtain is defined as a poly(dimethylsiloxane) (PDMS) wall that extends from 
      the ceiling of a cell culture microchamber to within microns of the chamber 
      floor. Curtain use is demonstrated by observing monolayer human epidermal 
      keratinocyte (HEK) colonies for 48 h longer than possible with non-curtained 
      microfluidic chambers. The curtains were further characterized by integrating 
      them into a 96 chamber high throughput microfluidic cell culture device. As proof 
      of concept, this device was used to assay a range of ethanol dilutions spanning 
      0-22% in cell culture medium. Cells exposed to 12% ethanol or less for 30 min 
      would recover to 85% viability at 24 h, while cells exposed to higher 
      concentrations had viabilities below 10%. The data also showed that cells exposed 
      to 6% ethanol or less grew in population size, 8% ethanol exposure stunted 
      growth, and higher concentrations led to population loss. Curtain use permitted 
      high initial cell seeding densities and increased the amount of time cells can be 
      cultured compared to multi-well plates.
FAU - O'Neill, Adrian T
AU  - O'Neill AT
AD  - Joint Department of Biomedical Engineering at The University of North Carolina at 
      Chapel Hill and North Carolina State University, Raleigh, NC 27695-7115, USA.
FAU - Monteiro-Riviere, Nancy A
AU  - Monteiro-Riviere NA
FAU - Walker, Glenn M
AU  - Walker GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090320
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Cytotoxins)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Nylons)
RN  - 0 (poly(dimethylsiloxane)-polyamide copolymer)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Cell Culture Techniques/*instrumentation
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cell Survival/drug effects
MH  - Collagen/metabolism
MH  - Cytotoxins/toxicity
MH  - Dimethylpolysiloxanes
MH  - Humans
MH  - Keratinocytes/cytology/drug effects
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Nylons
MH  - Skin/cytology
MH  - Time Factors
EDAT- 2009/06/06 09:00
MHDA- 2009/07/30 09:00
CRDT- 2009/06/05 09:00
PHST- 2009/06/05 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/07/30 09:00 [medline]
AID - 10.1039/b819622b [doi]
PST - ppublish
SO  - Lab Chip. 2009 Jun 21;9(12):1756-62. doi: 10.1039/b819622b. Epub 2009 Mar 20.