PMID- 19495464
OWN - NLM
STAT- MEDLINE
DCOM- 20090729
LR  - 20131121
IS  - 1473-0197 (Print)
IS  - 1473-0189 (Linking)
VI  - 9
IP  - 12
DP  - 2009 Jun 21
TI  - Directing polyallylamine adsorption on microlens array patterned silicon for 
      microarray fabrication.
PG  - 1789-96
LID - 10.1039/b900748m [doi]
AB  - The selective adsorption of reagents is often essential for bioarray and 
      lab-on-a-chip type devices. As the starting point for a bioarray, alkyl monolayer 
      terminated silicon shards were photopatterned in a few nanoseconds with thousands 
      of wells (spots) using an optical element, a microlens array. Polyallylamine 
      (PAAm), a primary amine containing polymer, adsorbed with little selectivity to 
      the spots, i.e., silicon oxide, over the hydrophobic background. However, at 
      appropriate concentrations, addition of a cationic surfactant to the PAAm 
      deposition solution, cetyltrimethylammonium chloride, prevented the nonspecific 
      adsorption of PAAm onto the hydrophobic monolayer, while directing it effectively 
      to the active spots on the device. A nonionic surfactant was less effective in 
      preventing the nonspecific adsorption of PAAm onto the hydrophobic monolayer. The 
      localized reactions/interactions of adsorbed PAAm with four species that are 
      useful for bioconjugate chemistry: glutaric anhydride, phenylenediisothiocyanate, 
      biotin NHS ester, and an oligonucleotide (DNA) were shown in the spots of an 
      array. The reactivity of PAAm was further demonstrated with an isocyanate. 
      Time-of-flight secondary ion mass spectrometry (ToF-SIMS) played an important 
      role in confirming selective surface reactivity and adsorption. X-ray 
      photoelectron spectroscopy (XPS), spectroscopic ellipsometry, and wetting 
      confirmed PAAm reactivity on planar substrates.
FAU - Saini, Gaurav
AU  - Saini G
AD  - Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 
      84602, USA.
FAU - Gates, Richard
AU  - Gates R
FAU - Asplund, Matthew C
AU  - Asplund MC
FAU - Blair, Steve
AU  - Blair S
FAU - Attavar, Sachin
AU  - Attavar S
FAU - Linford, Matthew R
AU  - Linford MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090316
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Polyamines)
RN  - 30551-89-4 (polyallylamine)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Adsorption
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Microarray Analysis/instrumentation/*methods
MH  - Microtechnology/*methods
MH  - Polyamines/*chemistry
MH  - Silicon/*chemistry
MH  - Silicon Dioxide/chemistry
MH  - Spectrum Analysis
MH  - Surface Properties
EDAT- 2009/06/06 09:00
MHDA- 2009/07/30 09:00
CRDT- 2009/06/05 09:00
PHST- 2009/06/05 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/07/30 09:00 [medline]
AID - 10.1039/b900748m [doi]
PST - ppublish
SO  - Lab Chip. 2009 Jun 21;9(12):1789-96. doi: 10.1039/b900748m. Epub 2009 Mar 16.