PMID- 19495708
OWN - NLM
STAT- MEDLINE
DCOM- 20090903
LR  - 20131121
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 550
DP  - 2009
TI  - Assessment of embryotoxicity using mouse embryo culture.
PG  - 241-9
LID - 10.1007/978-1-60327-009-0_15 [doi]
AB  - The mouse embryo culture technique is a valuable tool for assessing 
      embryotoxicity of exogenous compounds as it excludes any confounding maternal and 
      placental effects, allows for the selection of embryos that are at similar stages 
      of development, and permits the control of exposure concentrations of exogenous 
      agents and modifiers of interest. This chapter will use the anticonvulsant drug 
      valproic acid as a model teratogen to describe the mouse embryo culture in 
      detail. Briefly, mice are bred and the presence of a vaginal plug in a female 
      mouse indicates gestational day (GD) 1. On GD 9 embryos are explanted from 
      pregnant dams and embryos that are at similar stages of development (4-6 somite 
      pairs) are cultured in CO(2) saturated male rat serum for 24 h at 37 degrees C. 
      After 24 h embryonic morphological and developmental parameters, including 
      anterior neuropore closure, are evaluated using a dissecting microscope. 
      Additional biochemical analysis, including molecular approaches to assess 
      embryonic signal transduction, as well as some limitations of the technique will 
      also be discussed.
FAU - Winn, Louise M
AU  - Winn LM
AD  - Department of Pharmacology and School of Environmental Studies, Queen's 
      University, Kingston, Ontario, Canada.
FAU - Tung, Emily W Y
AU  - Tung EW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Anticonvulsants)
RN  - 0 (Culture Media)
RN  - 0 (Teratogens)
RN  - 614OI1Z5WI (Valproic Acid)
SB  - IM
MH  - Animals
MH  - Anticonvulsants/*toxicity
MH  - Culture Media
MH  - Embryo, Mammalian/*drug effects
MH  - Female
MH  - Mice
MH  - Pregnancy
MH  - Signal Transduction/drug effects
MH  - Teratogens/*toxicity
MH  - Valproic Acid/*toxicity
EDAT- 2009/06/06 09:00
MHDA- 2009/09/04 06:00
CRDT- 2009/06/05 09:00
PHST- 2009/06/05 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/09/04 06:00 [medline]
AID - 10.1007/978-1-60327-009-0_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;550:241-9. doi: 10.1007/978-1-60327-009-0_15.