PMID- 19495791
OWN - NLM
STAT- MEDLINE
DCOM- 20090731
LR  - 20211020
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 455
IP  - 1
DP  - 2009 Jul
TI  - RET/PTC rearrangements arising from a small population of papillary thyroid 
      carcinoma cells, possible candidate for passenger mutation.
PG  - 35-41
LID - 10.1007/s00428-009-0789-8 [doi]
AB  - RET rearrangements (RET/PTC) is a major genetic alteration in papillary thyroid 
      carcinomas. However, the prevalence of RET/PTC differs considerably among 
      investigators, and its impact on cancer progression has been controversial. In 
      the current study, we applied interphase fluorescence in situ hybridization 
      (FISH) to touch imprint cytology of 14 papillary thyroid carcinomas along with 
      reverse-transcription polymerase chain reaction (RT-PCR) analysis. FISH DNA 
      probes included RET locus, and PCR primers were designed targeting RET/PTC1 or 
      RET/PTC3. Split FISH signals of RET was observed in 78.6% (11/14) of tumors. 
      Proportions of tumor cells having split RET signals ranged from 1.8% to 19.6% 
      (mean 9.7%) in those 11 tumors. In RT-PCR analysis, RET/PTC was found in 28.6% 
      (4/14) of tumors. Among tumors with split RET signals, 36.4% (4/11) of tumors 
      exhibited detectable messenger RNA of RET/PTC1 or RET/PTC3. The remaining seven 
      tumors with split RET signals had no RET/PTCs amplicon. In conclusion, the 
      current study disclosed that RET/PTCs occur in a small population of tumor cells 
      in papillary thyroid carcinomas. Even though RET/PTC is a specific genetic event 
      in the carcinomas, our results suggested the possibility of RET/PTC as 
      "passenger" abnormalities rather than "driver" oncogenic mutation during thyroid 
      cancer progression, warranting further studies on mechanisms and implication of 
      RET gene instability.
FAU - Nakazawa, Tadao
AU  - Nakazawa T
AD  - Department of Pathology, University of Yamanashi Interdisciplinary Graduate 
      School of Medicine and Engineering, 1110 Shimokato, Chuo, Yamanashi, 409-3898, 
      Japan. tadaon@yamanashi.ac.jp
FAU - Murata, Shin-ichi
AU  - Murata S
FAU - Kondo, Tetsuo
AU  - Kondo T
FAU - Niu, Dongfeng
AU  - Niu D
FAU - Mochizuki, Kunio
AU  - Mochizuki K
FAU - Kawasaki, Tomonori
AU  - Kawasaki T
FAU - Yamane, Tetsu
AU  - Yamane T
FAU - Nakamura, Nobuki
AU  - Nakamura N
FAU - Katoh, Ryohei
AU  - Katoh R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090603
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Histones)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (ret-PTC fusion oncoproteins, human)
SB  - IM
MH  - Adult
MH  - Carcinoma, Papillary/*genetics
MH  - Chromosomes, Human, Pair 10
MH  - Female
MH  - *Gene Rearrangement
MH  - Histones/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - *Mutation
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Thyroid Neoplasms/*genetics
EDAT- 2009/06/06 09:00
MHDA- 2009/08/01 09:00
CRDT- 2009/06/05 09:00
PHST- 2009/03/02 00:00 [received]
PHST- 2009/05/09 00:00 [accepted]
PHST- 2009/05/06 00:00 [revised]
PHST- 2009/06/05 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/08/01 09:00 [medline]
AID - 10.1007/s00428-009-0789-8 [doi]
PST - ppublish
SO  - Virchows Arch. 2009 Jul;455(1):35-41. doi: 10.1007/s00428-009-0789-8. Epub 2009 
      Jun 3.