PMID- 19496975
OWN - NLM
STAT- MEDLINE
DCOM- 20100517
LR  - 20161125
IS  - 1442-200X (Electronic)
IS  - 1328-8067 (Linking)
VI  - 52
IP  - 1
DP  - 2010 Feb
TI  - Effect of vitamin C on oxidative liver injury due to isoniazid in rats.
PG  - 69-74
LID - 10.1111/j.1442-200X.2009.02891.x [doi]
AB  - BACKGROUND: The aim of the present study was to investigate the effect of 
      different doses of vitamin C on oxidative liver injury due to isoniazid (INH) in 
      rats. METHODS: Rats were divided into four subgroups, each containing 10 rats. 
      Group 1 was the control group; group 2, INH 50 mg/kg per day; group 3, INH 50 
      mg/kg per day + low-dose vitamin C (100 mg/kg per day); group 4, INH 50 mg/kg per 
      day + high-dose vitamin C (1000 mg/kg per day). INH and vitamin C were 
      administered into their stomachs through an oral tube. After 21 days, 
      measurements were made in both serum and homogenized liver tissues. The levels of 
      glutathione (GSH), superoxide dismutase (SOD) and other biochemical variables 
      were measured. Malondialdehyde (MDA), glutathione peroxidase (GSH-px) and vitamin 
      C were measured using commercial kits. RESULTS: Aspartate amino transferase and 
      alanine aminotransferase in group 2 were higher than those in groups 1, 3 and 4 
      (P < 0.008 for both). Serum and tissue levels of MDA in group 2 were higher than 
      that in groups 1 and 3 (P < 0.008 for both). There was no difference in the SOD 
      levels between the four groups (P= 0.095). Erythrocyte and tissue GSH in group 2 
      were higher than that in groups 1 and 3 (P < 0.008 for both). Interestingly, 
      erythrocyte and tissue GSH in group 4 were lower than those in group 1 (P < 0.008 
      for both). Erythrocyte level of GSH-px in group 2 was higher than that in groups 
      1 and 3 (P < 0.008 for both). CONCLUSIONS: INH-induced liver injury is associated 
      with oxidative stress, and co-administration of low-dose vitamin C may reduce 
      this damage effectively in a rat model. The antioxidant effect of high-dose 
      vitamin C does not seem more potent compared to the low dose.
FAU - Ergul, Yakup
AU  - Ergul Y
AD  - Department of Pediatrics, Cerrahpasa Medical School, Istanbul University, 
      Istanbul, Turkey. yakupergul77@hotmail.com
FAU - Erkan, Tulay
AU  - Erkan T
FAU - Uzun, Hafize
AU  - Uzun H
FAU - Genc, Habibe
AU  - Genc H
FAU - Altug, Tuncay
AU  - Altug T
FAU - Erginoz, Ethem
AU  - Erginoz E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090601
PL  - Australia
TA  - Pediatr Int
JT  - Pediatrics international : official journal of the Japan Pediatric Society
JID - 100886002
RN  - 0 (Antioxidants)
RN  - 0 (Antitubercular Agents)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - V83O1VOZ8L (Isoniazid)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Antitubercular Agents/*toxicity
MH  - Ascorbic Acid/blood/*pharmacology
MH  - Chemical and Drug Induced Liver Injury/*physiopathology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Glutathione/blood
MH  - Glutathione Peroxidase/blood
MH  - Isoniazid/*toxicity
MH  - Liver Function Tests
MH  - Male
MH  - Malondialdehyde/blood
MH  - Oxidative Stress/*drug effects/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/blood
EDAT- 2009/06/06 09:00
MHDA- 2010/05/18 06:00
CRDT- 2009/06/06 09:00
PHST- 2009/06/06 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2010/05/18 06:00 [medline]
AID - PED2891 [pii]
AID - 10.1111/j.1442-200X.2009.02891.x [doi]
PST - ppublish
SO  - Pediatr Int. 2010 Feb;52(1):69-74. doi: 10.1111/j.1442-200X.2009.02891.x. Epub 
      2009 Jun 1.