PMID- 19497022
OWN - NLM
STAT- MEDLINE
DCOM- 20110602
LR  - 20101122
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 45
IP  - 6
DP  - 2010 Dec
TI  - Detection of sex chromosome aneuploidy in equine spermatozoa using fluorescence 
      in situ hybridization.
PG  - 1015-9
LID - 10.1111/j.1439-0531.2009.01478.x [doi]
AB  - The aim of our study was to diagnose aneuploidy in equine spermatozoa by 
      multicolour fluorescence in situ hybridization (FISH) technique using specific 
      molecular probes for equine sex chromosomes and autosome pair four (EGFR probe) 
      labeled by different fluorochromes. These were applied on decondensed spermatozoa 
      of four stallions. In total, more than 8800 sperm cells were examined. The total 
      frequency of aberrant cells was 0.496%: aneuploidy of XX (0.135%), YY (0.023%), 
      XY (0.102%), diploidy (0.057%), lack of sex chromosome (0.18%). In one stallion 
      the ratio of normal X- and Y-bearing cells was different from the expected 1:1 
      ratio (p = 0.0002), in all three other stallions this ratio was close to 1:1. The 
      present study demonstrated that the FISH technique is a powerful method to 
      identify sex chromosome aberrations in equine spermatozoa and allows for the 
      determination of the ratio between X-Y-spermatozoa.
CI  - (c) 2009 Blackwell Verlag GmbH.
FAU - Bugno, M
AU  - Bugno M
AD  - National Research Institute of Animal Production, Department of Immuno- and 
      Cytogenetics, Krakowska, Balice, Poland. mbugno@izoo.krakow.pl
FAU - Jablonska, Z
AU  - Jablonska Z
FAU - Tischner, M
AU  - Tischner M
FAU - Klukowska-Rotzler, J
AU  - Klukowska-Rotzler J
FAU - Pienkowska-Schelling, A
AU  - Pienkowska-Schelling A
FAU - Schelling, C
AU  - Schelling C
FAU - Slota, E
AU  - Slota E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - Female
MH  - In Situ Hybridization, Fluorescence/*veterinary
MH  - Male
MH  - *Sex Chromosomes
MH  - Spermatozoa/*cytology
EDAT- 2009/06/06 09:00
MHDA- 2011/06/03 06:00
CRDT- 2009/06/06 09:00
PHST- 2009/06/06 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2011/06/03 06:00 [medline]
AID - RDA1478 [pii]
AID - 10.1111/j.1439-0531.2009.01478.x [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2010 Dec;45(6):1015-9. doi: 10.1111/j.1439-0531.2009.01478.x.