PMID- 19497040
OWN - NLM
STAT- MEDLINE
DCOM- 20091008
LR  - 20191210
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 74
IP  - 2
DP  - 2009 Aug
TI  - Correlations between Terasaki's HLA class II epitopes and HLAMatchmaker-defined 
      eplets on HLA-DR and -DQ antigens.
PG  - 134-46
LID - 10.1111/j.1399-0039.2009.01272.x [doi]
AB  - Human leukocyte antigen (HLA) class II-specific antibodies increase the risk of 
      transplant failure, and their characterization must consider epitopes rather than 
      antigens. There are two strategies to determine HLA epitope structure. Terasaki's 
      group has analyzed antibody reactivity patterns with single antigen panels with a 
      computer program based on shared amino acid residues of reactive alleles. 
      HLAMatchmaker is a theoretical algorithm that predicts HLA epitopes on the HLA 
      molecular surface from stereochemical modeling of epitope-paratope interfaces of 
      antigen-antibody complexes. Our epitope repertoire is based on so-called 'eplets' 
      representing 3-A patches of at least one polymorphic residue on the molecular 
      surface. This report describes how 49 of 53 Terasaki's HLA-DR epitopes correspond 
      to HLAMatchmaker-defined eplets. Most of them are equivalent to single eplets (n 
      = 33) or two or more possible eplets (n = 10), but six had corresponding eplet 
      pairs. There were 10 cases whereby eplets have permissible residue combinations, 
      and in 5 cases, we found that eplet specificity might be influenced by nearby 
      hidden residues. We could assign corresponding eplets to 17 of 18 Terasaki's 
      HLA-DQ epitopes. This study demonstrates how the HLAMatchmaker interpretation of 
      amino acid residues shared between antibody-reactive antigens can increase our 
      understanding of the structural basis of HLA epitopes.
FAU - Marrari, M
AU  - Marrari M
AD  - Division of Transplantation Pathology, Thomas E. Starzl Transplantation 
      Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA.
FAU - Duquesnoy, R J
AU  - Duquesnoy RJ
LA  - eng
GR  - R01 AI-55933/AI/NIAID NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090602
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Epitopes)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Immunodominant Epitopes)
SB  - IM
MH  - *Algorithms
MH  - Amino Acid Sequence
MH  - Antibody Specificity
MH  - Antigen-Antibody Complex/analysis/immunology
MH  - Epitope Mapping/*methods
MH  - Epitopes/*analysis/immunology
MH  - HLA-D Antigens/chemistry/*immunology
MH  - HLA-DQ Antigens/analysis/chemistry/immunology
MH  - HLA-DR Antigens/analysis/chemistry/immunology
MH  - Humans
MH  - Immunodominant Epitopes/analysis/chemistry
MH  - Models, Molecular
MH  - Sequence Analysis, Protein
MH  - Structure-Activity Relationship
EDAT- 2009/06/06 09:00
MHDA- 2009/10/09 06:00
CRDT- 2009/06/06 09:00
PHST- 2009/06/06 09:00 [entrez]
PHST- 2009/06/06 09:00 [pubmed]
PHST- 2009/10/09 06:00 [medline]
AID - TAN1272 [pii]
AID - 10.1111/j.1399-0039.2009.01272.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2009 Aug;74(2):134-46. doi: 10.1111/j.1399-0039.2009.01272.x. 
      Epub 2009 Jun 2.