PMID- 19502222
OWN - NLM
STAT- MEDLINE
DCOM- 20100218
LR  - 20151119
IS  - 1759-4685 (Electronic)
IS  - 1759-4685 (Linking)
VI  - 1
IP  - 1
DP  - 2009 Oct
TI  - Generation of pig induced pluripotent stem cells with a drug-inducible system.
PG  - 46-54
LID - 10.1093/jmcb/mjp003 [doi]
AB  - Domesticated ungulate pluripotent embryonic stem (ES) cell lines would be useful 
      for generating precise gene-modified animals. To date, many efforts have been 
      made to establish domesticated ungulate pluripotent ES cells from early embryos 
      without success. Here, we report the generation of porcine-induced pluripotent 
      stem (iPS) cells using drug-inducible expression of defined factors. We showed 
      that porcine iPS cells expressed alkaline phosphatase, SSEA3, SSEA4, Tra-1-60, 
      Tra-1-81, Oct3/4, Nanog, Sox2, Rex1 and CDH1. Pig iPS cells expressed high levels 
      of telomerase activity and showed normal karyotypes. These cells could 
      differentiate into cell types of all three germ layers in vitro and in teratomas. 
      Our study reveals properties of porcine pluripotent stem cells that may 
      facilitate the eventual establishment of porcine ES cells. Moreover, the porcine 
      iPS cells produced may be directly useful for the generation of precise 
      gene-modified pigs.
FAU - Wu, Zhao
AU  - Wu Z
AD  - Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological 
      Sciences, Chinese Academy of Sciences, People's Republic of China.
FAU - Chen, Jijun
AU  - Chen J
FAU - Ren, Jiangtao
AU  - Ren J
FAU - Bao, Lei
AU  - Bao L
FAU - Liao, Jing
AU  - Liao J
FAU - Cui, Chun
AU  - Cui C
FAU - Rao, Linjun
AU  - Rao L
FAU - Li, Hui
AU  - Li H
FAU - Gu, Yijun
AU  - Gu Y
FAU - Dai, Huiming
AU  - Dai H
FAU - Zhu, Hui
AU  - Zhu H
FAU - Teng, Xiaokun
AU  - Teng X
FAU - Cheng, Lu
AU  - Cheng L
FAU - Xiao, Lei
AU  - Xiao L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090603
PL  - United States
TA  - J Mol Cell Biol
JT  - Journal of molecular cell biology
JID - 101503669
RN  - 0 (Biomarkers)
RN  - N12000U13O (Doxycycline)
SB  - IM
MH  - Aging/physiology
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology/drug effects/metabolism
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Shape/drug effects
MH  - Cellular Reprogramming/drug effects
MH  - Cluster Analysis
MH  - Doxycycline/*pharmacology
MH  - Embryonic Stem Cells/cytology/drug effects/metabolism
MH  - Epigenesis, Genetic/drug effects
MH  - Fibroblasts/cytology/drug effects/metabolism
MH  - Gene Expression Profiling
MH  - Genetic Vectors/genetics
MH  - Germ Layers/cytology/drug effects/metabolism
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology/*drug effects/metabolism
MH  - Lentivirus/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - *Sus scrofa
MH  - Transduction, Genetic
EDAT- 2009/06/09 09:00
MHDA- 2010/02/19 06:00
CRDT- 2009/06/09 09:00
PHST- 2009/06/09 09:00 [entrez]
PHST- 2009/06/09 09:00 [pubmed]
PHST- 2010/02/19 06:00 [medline]
AID - mjp003 [pii]
AID - 10.1093/jmcb/mjp003 [doi]
PST - ppublish
SO  - J Mol Cell Biol. 2009 Oct;1(1):46-54. doi: 10.1093/jmcb/mjp003. Epub 2009 Jun 3.