PMID- 19504438
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20221207
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 26
IP  - 3
DP  - 2009 Jun
TI  - [Identification of a novel allele HLA-DRB1*1218].
PG  - 272-6
LID - 10.3760/cma.j.issn.1003-9406.2009.03.008 [doi]
AB  - OBJECTIVE: To identify a novel human leukocyte antigen (HLA) allele by cloning 
      and sequence-based typing in Chinese population, and analyzing the sequence of 
      the introns 1 and 2. METHODS: The routine HLA-A, -B, -DRB1 low resolution 
      genotyping for stem cell donor from Guangdong province was performed with 
      polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP). An 
      unknown HLA-DRB1 allele was initially detected by HLA typing. Genomic DNA of the 
      proband was amplified by using HLA-DRB1 locus group-specific primer, the 
      amplified product was cloned, sequenced, and compared to the closest DRB1*120201 
      allele and the closest intron sequence of the DRB1*030101 allele. RESULTS: The 
      sequencing results showed that a normal DRB1*080302 and a novel DRB1*1218 variant 
      allele were identified. The sequence of the novel allele has been submitted to 
      GenBank (FJ481086). The novel allele had 1 nucleotide substitution of the closest 
      matching allele HLA-DRB1*120201 at nt262(G-->C) in exon 2,resulting in an amino 
      acid change from Glu(GAG)-->Gln (CAG) at codon 59.The intron 2 sequence is 
      identical between the novel HLA-DRB1*1218 and DRB1*030101, but there are 12 
      nucleotides substitution in intron 1. CONCLUSION: A novel HLA allele was 
      confirmed by cloning and sequence-based typing in Chinese. It was officially 
      designated as HLA-DRB1*1218 by WHO Nomenclature Committee.
FAU - Gao, Su-qing
AU  - Gao SQ
AD  - Shenzhen Blood Center Institute, Shenzhen HLA Laboratory of CMDP, Shenzhen, 
      Guangdong, 518035 PR China.
FAU - Deng, Zhi-hui
AU  - Deng ZH
FAU - Xu, Yun-ping
AU  - Xu YP
LA  - chi
SI  - GENBANK/FJ481086
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0RH81L854J (Glutamine)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - *Alleles
MH  - *Amino Acid Substitution
MH  - Asian People/genetics
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Exons/genetics
MH  - Glutamic Acid/genetics
MH  - Glutamine/genetics
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Molecular Sequence Data
EDAT- 2009/06/09 09:00
MHDA- 2009/09/18 06:00
CRDT- 2009/06/09 09:00
PHST- 2009/06/09 09:00 [entrez]
PHST- 2009/06/09 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
AID - 940626058 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2009.03.008 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Jun;26(3):272-6. doi: 
      10.3760/cma.j.issn.1003-9406.2009.03.008.