PMID- 19505514
OWN - NLM
STAT- MEDLINE
DCOM- 20091117
LR  - 20090907
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 145
IP  - 1
DP  - 2009 Oct
TI  - Identification and characterization of a porcine monocytic cell line supporting 
      porcine reproductive and respiratory syndrome virus (PRRSV) replication and 
      progeny virion production by using an improved DNA-launched PRRSV reverse 
      genetics system.
PG  - 1-8
LID - 10.1016/j.virusres.2009.05.013 [doi]
AB  - In this study, an improved DNA-launched (plasmid DNA transfection-based) reverse 
      genetics system with reduced cost and labor was developed for porcine 
      reproductive and respiratory syndrome virus (PRRSV) by introduction of ribozyme 
      elements at both termini of the viral genomic cDNA that were placed under the 
      control of a eukaryotic hybrid promoter. The rescue efficacy of PRRSV with this 
      system was approximately 10-50-fold higher than the in vitro-transcribed 
      RNA-based system and the traditional DNA-launched system without the engineered 
      ribozyme elements, as determined by reporter GFP level in transfected cells and 
      the peak titer of the recovery virus. By using this new reverse genetics system, 
      we identified and characterized a porcine monocytic cell line, 3D4/31, capable of 
      supporting PRRSV replication, progeny virion production, and attachment on the 
      cell surface. The establishment of this improved reverse genetic system and the 
      identification of a porcine monocytic cell line supporting PRRSV replication will 
      aid future studies of host-virus interaction of PRRSV.
FAU - Huang, Y W
AU  - Huang YW
AD  - Center for Molecular Medicine and Infectious Diseases, Department of Biomedical 
      Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA 24061-0342, USA.
FAU - Fang, Y
AU  - Fang Y
FAU - Meng, X J
AU  - Meng XJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090606
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Recombinant)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chickens
MH  - DNA, Recombinant/genetics/metabolism
MH  - DNA, Viral/genetics/metabolism
MH  - Host-Pathogen Interactions
MH  - Porcine Reproductive and Respiratory Syndrome/metabolism/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - Transfection/methods
MH  - Virion/*metabolism
MH  - Virus Attachment
MH  - Virus Internalization
MH  - *Virus Replication
EDAT- 2009/06/10 09:00
MHDA- 2009/11/18 06:00
CRDT- 2009/06/10 09:00
PHST- 2009/02/12 00:00 [received]
PHST- 2009/05/26 00:00 [revised]
PHST- 2009/05/28 00:00 [accepted]
PHST- 2009/06/10 09:00 [entrez]
PHST- 2009/06/10 09:00 [pubmed]
PHST- 2009/11/18 06:00 [medline]
AID - S0168-1702(09)00212-3 [pii]
AID - 10.1016/j.virusres.2009.05.013 [doi]
PST - ppublish
SO  - Virus Res. 2009 Oct;145(1):1-8. doi: 10.1016/j.virusres.2009.05.013. Epub 2009 
      Jun 6.