PMID- 19505688
OWN - NLM
STAT- MEDLINE
DCOM- 20100901
LR  - 20220311
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 94
IP  - 3
DP  - 2010 Aug
TI  - Numerical chromosome abnormalities are associated with sperm tail swelling 
      patterns.
PG  - 1012-20
LID - 10.1016/j.fertnstert.2009.04.043 [doi]
AB  - OBJECTIVE: To assess whether the differential sperm tail swelling patterns 
      observed following hypo-osmotic shock are useful in discriminating normal sperm 
      from aneuploid sperm. DESIGN: Prospective study. SETTING: University research 
      setting. INTERVENTION(S): Fluorescence in situ hybridization (FISH) was combined 
      with hypo-osmotic swelling for a simultaneous assessment of aneuploidy and 
      viability in human spermatozoa. MAIN OUTCOME MEASURE(S): FISH for chromosomes 1, 
      13, 18, 21, X, and Y after hypo-osmotic stress was used to investigate the 
      distribution of sperm aneuploidy related to sperm-tail swelling patterns. A total 
      of 16,473 sperm cells were scored from three normal fertile donors and six 
      oligoasthenoteratozoospermic (OAT) patients. RESULT(S): There was a 17.2-fold 
      decrease in the frequency of total aneuploidy in the sperm with a tail-tip 
      swelling pattern compared with the initial nonselected sperm in the OAT patients. 
      Strikingly, when the sperm with tail-tip swelling patterns were screened from the 
      patients, the frequency of total aneuploidy was actually lower by a factor of 
      four than in the nonselected sperm from fertile donors. CONCLUSION(S): The sperm 
      cells with tail-tip swelling patterns are related to a low frequency of 
      aneuploidy.
CI  - Copyright (c) 2010 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Pang, Myung-Geol
AU  - Pang MG
AD  - Department of Animal Science and Technology and BET Research Institute, Chung-Ang 
      University, Ansung, Gyeonggi-Do, South Korea. mgpang@cau.ac.kr
FAU - You, Young-Ah
AU  - You YA
FAU - Park, Yoo-Jin
AU  - Park YJ
FAU - Oh, Shin-Ae
AU  - Oh SA
FAU - Kim, Dai-Sik
AU  - Kim DS
FAU - Kim, Young-Ju
AU  - Kim YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090607
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Adsorption
MH  - Aneuploidy
MH  - Case-Control Studies
MH  - Cell Survival/physiology
MH  - *Chromosome Aberrations
MH  - Chromosome Disorders/diagnosis/genetics/pathology
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 21
MH  - *DNA Copy Number Variations/physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/genetics
MH  - Male
MH  - Osmotic Pressure/*physiology
MH  - Sperm Tail/metabolism/*pathology
MH  - Water/metabolism
EDAT- 2009/06/10 09:00
MHDA- 2010/09/02 06:00
CRDT- 2009/06/10 09:00
PHST- 2009/02/09 00:00 [received]
PHST- 2009/04/07 00:00 [revised]
PHST- 2009/04/13 00:00 [accepted]
PHST- 2009/06/10 09:00 [entrez]
PHST- 2009/06/10 09:00 [pubmed]
PHST- 2010/09/02 06:00 [medline]
AID - S0015-0282(09)01011-5 [pii]
AID - 10.1016/j.fertnstert.2009.04.043 [doi]
PST - ppublish
SO  - Fertil Steril. 2010 Aug;94(3):1012-20. doi: 10.1016/j.fertnstert.2009.04.043. 
      Epub 2009 Jun 7.