PMID- 19505732
OWN - NLM
STAT- MEDLINE
DCOM- 20100205
LR  - 20141120
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 132
IP  - 2-4
DP  - 2009 Dec 15
TI  - Chicken heterophils from commercially selected and non-selected genetic lines 
      express cytokines differently after in vitro exposure to Salmonella enteritidis.
PG  - 129-34
LID - 10.1016/j.vetimm.2009.05.010 [doi]
AB  - Resistance to pathogens such as Salmonella enteritidis (SE) is a heritable trait 
      important in maintaining the health of chickens and reducing bacterial 
      contamination of poultry products. In chickens, heterophils act as the first 
      responders to bacterial infections and are, therefore, responsible for initiating 
      the immune response against SE challenge. This study measured mRNA expression of 
      several immune response genes [interleukin-6 (IL-6), IL-10, transforming growth 
      factor-beta4 (TGF-beta4), granulocyte macrophage-colony stimulating factor 
      (GM-CSF), and Toll-like receptor-4 (TLR-4)] by heterophils from broiler, Leghorn, 
      and Fayoumi chickens, either non-stimulated or stimulated in vitro with SE using 
      quantitative reverse-transcriptase PCR. We found that heterophils of commercially 
      selected broiler and Leghorn birds had differing early heterophil responses to SE 
      in comparison with the native Fayoumi line. Heterophil stimulation with SE in 
      vitro increased expression of pro- (IL-6 and GM-CSF) and anti-inflammatory 
      cytokine mRNA (IL-10 and TGF-beta4) in the Fayoumi line, while the broiler and 
      Leghorn line heterophils had decreased or no changes in the cytokine gene 
      expression levels. The unique response of the Fayoumi line is in contrast to the 
      lines with a history of genetic selection to increase growth or reproduction, a 
      process which may favor reduced or suppressed inflammatory responses. The 
      findings illustrate the potential value of native lines to provide biodiversity 
      to enhance innate health in commercially selected poultry.
FAU - Redmond, Sarah B
AU  - Redmond SB
AD  - Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, IA 
      50011, United States.
FAU - Chuammitri, Phongsakorn
AU  - Chuammitri P
FAU - Andreasen, Claire B
AU  - Andreasen CB
FAU - Palic, Dusan
AU  - Palic D
FAU - Lamont, Susan J
AU  - Lamont SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090520
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (transforming growth factor beta4)
RN  - 130068-27-8 (Interleukin-10)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Chickens/blood/*genetics/*immunology/microbiology
MH  - Cytokines/*genetics
MH  - Gene Expression
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics
MH  - Granulocytes/*immunology/microbiology
MH  - In Vitro Techniques
MH  - Interleukin-10/genetics
MH  - Interleukin-6/genetics
MH  - Poultry Diseases/genetics/immunology
MH  - Quantitative Trait, Heritable
MH  - RNA, Messenger/genetics/metabolism
MH  - Salmonella Infections, Animal/genetics/immunology
MH  - Salmonella enteritidis/*immunology/*pathogenicity
MH  - Species Specificity
MH  - Toll-Like Receptor 4/genetics
MH  - Transforming Growth Factor beta/genetics
EDAT- 2009/06/10 09:00
MHDA- 2010/02/06 06:00
CRDT- 2009/06/10 09:00
PHST- 2009/02/10 00:00 [received]
PHST- 2009/04/14 00:00 [revised]
PHST- 2009/05/11 00:00 [accepted]
PHST- 2009/06/10 09:00 [entrez]
PHST- 2009/06/10 09:00 [pubmed]
PHST- 2010/02/06 06:00 [medline]
AID - S0165-2427(09)00170-6 [pii]
AID - 10.1016/j.vetimm.2009.05.010 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2009 Dec 15;132(2-4):129-34. doi: 
      10.1016/j.vetimm.2009.05.010. Epub 2009 May 20.