PMID- 19508364
OWN - NLM
STAT- MEDLINE
DCOM- 20090629
LR  - 20090610
IS  - 1399-3054 (Electronic)
IS  - 0031-9317 (Linking)
VI  - 136
IP  - 1
DP  - 2009 May
TI  - Promoter deletion analysis elucidates the role of cis elements and 5'UTR intron 
      in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis.
PG  - 10-8
LID - 10.1111/j.1399-3054.2009.01207.x [doi]
AB  - The high-affinity phosphate transporter AtPht1;4 (Arabidopsis phosphate 
      transporter1;4) is not only induced in response to inorganic phosphate (Pi) 
      starvation but also preferentially expressed in the roots of Arabidopsis. In this 
      study, we carried out AtPht1;4 promoter deletion analysis to identify regions 
      that control the Pi responsiveness and spatiotemporal expression of the gene. 
      Expression cassettes with truncated promoter fragments cloned to GUS 
      (beta-glucuronidase) coding sequence were developed. Full-length promoter (-2327) 
      and truncations up to -1436 (from the translational start) showed normal 
      expression of GUS in various parts of the plants. The Pi responsiveness and 
      inducibility of the reporter gene remained unaltered. However, deletion of the 
      promoter region containing the first PHR1-binding site (P1BS) motif (-1350) 
      abolished the AtPht1;4 expression in roots but not in aerial parts. A 164-bp 
      region immediately upstream of the transcription start site appears to be 
      sufficient for the basal expression of the gene. Interestingly, the 5'UTR (5' 
      untranslated region) intron exhibited weak promoter activity as evidenced by its 
      ability to drive the expression of AtPht1;4 in stipules and reproductive organs. 
      Further analyses showed that the 5'UTR intron is essential for AtPht1;4 
      expression in root tips besides enhancing the level of expression in roots during 
      Pi starvation. However, expression of AtPht1;4 in aerial parts of the plant was 
      not influenced by the intron. Together these results suggest that expression of 
      AtPht1;4 in the roots and aerial parts is regulated by independent mechanisms.
FAU - Karthikeyan, Athikkattuvalasu S
AU  - Karthikeyan AS
AD  - Department of Horticulture and Landscape Architecture, Purdue University, West 
      Lafayette, IN 47907, USA.
FAU - Ballachanda, Devaiah N
AU  - Ballachanda DN
FAU - Raghothama, Kashchandra G
AU  - Raghothama KG
LA  - eng
PT  - Journal Article
DEP - 20090204
PL  - Denmark
TA  - Physiol Plant
JT  - Physiologia plantarum
JID - 1256322
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (PHR1 protein, Arabidopsis)
RN  - 0 (PT2 protein, Arabidopsis)
RN  - 0 (Phosphate Transport Proteins)
RN  - 0 (Phosphates)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Arabidopsis/*genetics/metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Base Sequence
MH  - Gene Expression Regulation, Plant
MH  - Introns
MH  - Molecular Sequence Data
MH  - Phosphate Transport Proteins/genetics/*metabolism
MH  - Phosphates/metabolism
MH  - Plant Roots/genetics/metabolism
MH  - Plants, Genetically Modified/genetics/metabolism
MH  - *Promoter Regions, Genetic
MH  - Transcription Factors/metabolism
EDAT- 2009/06/11 09:00
MHDA- 2009/06/30 09:00
CRDT- 2009/06/11 09:00
PHST- 2009/06/11 09:00 [entrez]
PHST- 2009/06/11 09:00 [pubmed]
PHST- 2009/06/30 09:00 [medline]
AID - PPL1207 [pii]
AID - 10.1111/j.1399-3054.2009.01207.x [doi]
PST - ppublish
SO  - Physiol Plant. 2009 May;136(1):10-8. doi: 10.1111/j.1399-3054.2009.01207.x. Epub 
      2009 Feb 4.