PMID- 19513624
OWN - NLM
STAT- MEDLINE
DCOM- 20101021
LR  - 20221207
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 29
IP  - 3
DP  - 2009 Jun
TI  - Clinical significance of hTERC gene amplification detection by FISH in the 
      screening of cervical lesions.
PG  - 368-71
LID - 10.1007/s11596-009-0321-z [doi]
AB  - This study evaluated the clinical significance of hTERC gene amplification 
      detection by fluorescence in situ hybridization (FISH) in the screening of 
      cervical lesions. Cervical specimens of 50 high risk patients were detected by 
      thin liquid-based cytology. The patients whose cytological results were 
      classified as ASCUS or above were subjected to the subsequent colposcopic 
      biopsies. Slides prepared from these 50 cervical specimens were analyzed for 
      hTERC gene amplification using interphase FISH with the two-color hTERC probe. 
      The results of the cytological analysis and those of subsequent biopsies, when 
      available, were compared with the FISH-detected hTERC abnormalities. It was found 
      that the positive rates of hTERC gene amplification in NILM, ASCUS, LSIL, HSIL, 
      and SCC groups were 0.00, 28.57%, 57.14%, 100%, and 100%, respectively. The 
      positive rates of hTERC gene amplification in HSIL and SCC groups were 
      significantly higher than those in NILM, ASCUS and LSIL groups (all P<0.05). The 
      mean percentages of cells with hTERC gene amplification in NILM, ASCUS, LSIL, 
      HSIL, and SCC groups were 0.00, 10.50%, 36.00%, 79.00%, and 96.50%, respectively. 
      Patients with HSIL or SCC cytological diagnoses had significantly higher mean 
      percentages of cells with hTERC gene amplification than did patients with NILM, 
      ASCUS or LSIL cytological diagnoses (all P<0.05). It was concluded that two-color 
      interphase FISH could detect hTERC gene amplification to accurately distinguish 
      HSIL and ISIL of cervical cells. It may be an adjunct to cytology screening, 
      especially high-risk patients.
FAU - Zhang, Yuan
AU  - Zhang Y
AD  - Department of Gynaecology and Obstetrics, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430022, China. 
      yuanzhang75@163.com
FAU - Wang, Xiaobei
AU  - Wang X
FAU - Ma, Ling
AU  - Ma L
FAU - Wang, Zehua
AU  - Wang Z
FAU - Hu, Lihua
AU  - Hu L
LA  - eng
PT  - Journal Article
DEP - 20090610
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - Cervix Uteri/metabolism/*pathology
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Diseases/genetics/pathology
MH  - Uterine Cervical Neoplasms/*genetics/pathology
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/*genetics/pathology
EDAT- 2009/06/11 09:00
MHDA- 2010/10/22 06:00
CRDT- 2009/06/11 09:00
PHST- 2008/12/02 00:00 [received]
PHST- 2009/06/11 09:00 [entrez]
PHST- 2009/06/11 09:00 [pubmed]
PHST- 2010/10/22 06:00 [medline]
AID - 10.1007/s11596-009-0321-z [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2009 Jun;29(3):368-71. doi: 
      10.1007/s11596-009-0321-z. Epub 2009 Jun 10.