PMID- 19515021
OWN - NLM
STAT- MEDLINE
DCOM- 20090915
LR  - 20090611
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 44
IP  - 1
DP  - 2009 Feb
TI  - Human leukocyte histocompatibility antigen class II-induced cytokines from human 
      gingival fibroblasts promote proliferation of human umbilical vein endothelial 
      cells: potential association with enhanced angiogenesis in chronic periodontal 
      inflammation.
PG  - 103-9
LID - 10.1111/j.1600-0765.2008.01097.x [doi]
AB  - BACKGROUND AND OBJECTIVE: The role of human leukocyte histocompatibility antigen 
      (HLA) class II molecules on non-antigen-presenting cells has been a matter of 
      controversy. We previously reported that HLA-II molecules on human gingival 
      fibroblasts (GF) do not present antigens, but transduce signals into the cells, 
      resulting in the expression of several cytokines, such as interleukin-6 (IL-6), 
      monocyte chemoattractant protein-1 (MCP-1), regulated upon activation, normal 
      T-cell expressed and secreted (RANTES) and IL-8. However, the exact role of these 
      cytokines, as well as other cytokines which are potentially secreted from GF, in 
      the pathogenesis of chronic periodontal inflammation is not fully understood. The 
      aim of this study was to observe the effects of HLA-II-induced cytokines on the 
      proliferation of human umbilical vein endothelial cells (HUVEC). MATERIAL AND 
      METHODS: Antibody-based cytokine-microarray analyses were performed to detect 
      potential cytokines associated with angiogenesis. Next, cytokine productivity was 
      confirmed by quantitative methods. Then, cell proliferation assay was performed 
      to see whether these cytokines promoted the proliferation of HUVEC. RESULTS: 
      Besides IL-6, MCP-1, RANTES and IL-8, growth-related gene product (GRO) was newly 
      identified as an HLA-II-induced cytokine released from GF. This was confirmed by 
      a quantitative method. Cell culture supernatant from HLA-II-stimulated GF 
      cultures promoted the growth of HUVEC. Addition of anti-IL-8 neutralizing 
      antibody, anti-CXC receptor (CXCR)1 antibody and anti-MCP-1 antibody inhibited 
      the growth of HUVEC in a dose-dependent manner, while addition of anti-GROalpha 
      antibody did not. CONCLUSION: The HLA-II-induced IL-8, via CXCR1, as well as 
      MCP-1 from GF, promotes endothelial cell proliferation, which is possibly 
      associated with enhanced angiogenesis in chronic periodontal lesions.
FAU - Okada, Y
AU  - Okada Y
AD  - Department of Patho-physiology-Periodontal Science, Okayama University Graduate 
      School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
FAU - Meguro, M
AU  - Meguro M
FAU - Ohyama, H
AU  - Ohyama H
FAU - Yoshizawa, S
AU  - Yoshizawa S
FAU - Takeuchi-Hatanaka, K
AU  - Takeuchi-Hatanaka K
FAU - Kato, N
AU  - Kato N
FAU - Matsushita, S
AU  - Matsushita S
FAU - Takashiba, S
AU  - Takashiba S
FAU - Nishimura, F
AU  - Nishimura F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Antibodies)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Cytokines)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Receptors, Interleukin-8A)
SB  - IM
MH  - Antibodies/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokine CCL2/antagonists & inhibitors/immunology
MH  - Chemokine CCL5/immunology
MH  - Chemokine CXCL1/immunology
MH  - Chronic Periodontitis/immunology/*pathology
MH  - Cytokines/*immunology
MH  - Endothelial Cells/immunology/*pathology
MH  - Endothelium, Vascular/immunology/*pathology
MH  - Fibroblasts/*immunology
MH  - Gingiva/*immunology/pathology
MH  - HLA-DQ Antigens/immunology
MH  - HLA-DR Antigens/immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Humans
MH  - Interleukin-6/immunology
MH  - Interleukin-8/antagonists & inhibitors/immunology
MH  - Neovascularization, Pathologic/immunology/*pathology
MH  - Receptors, Interleukin-8A/antagonists & inhibitors/immunology
MH  - Umbilical Veins/immunology/*pathology
EDAT- 2009/06/12 09:00
MHDA- 2009/09/16 06:00
CRDT- 2009/06/12 09:00
PHST- 2009/06/12 09:00 [entrez]
PHST- 2009/06/12 09:00 [pubmed]
PHST- 2009/09/16 06:00 [medline]
AID - JRE1097 [pii]
AID - 10.1111/j.1600-0765.2008.01097.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2009 Feb;44(1):103-9. doi: 10.1111/j.1600-0765.2008.01097.x.