PMID- 19515200
OWN - NLM
STAT- MEDLINE
DCOM- 20091021
LR  - 20131121
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 11
IP  - 9
DP  - 2009 Sep
TI  - Ammonia-oxidizing communities in a highly aerated full-scale activated sludge 
      bioreactor: betaproteobacterial dynamics and low relative abundance of 
      Crenarchaea.
PG  - 2310-28
LID - 10.1111/j.1462-2920.2009.01958.x [doi]
AB  - Ammonia-oxidizing bacteria (AOB) have long been considered key to the removal of 
      nitrogen in activated sludge bioreactors. Culture-independent molecular analyses 
      have established that AOB lineages in bioreactors are dynamic, but the underlying 
      operational or environmental factors are unclear. Furthermore, the contribution 
      of ammonia-oxidizing archaea (AOA) to nitrogen removal in bioreactors has not 
      been studied. To this end, we investigated the abundance of AOA and AOB as well 
      as correlations between dynamics in AOB lineages and operational parameters at a 
      municipal wastewater treatment plant sampled weekly over a 1 year period. 
      Quantitative PCR measurements of bacterial and archaeal ammonia monooxygenase 
      subunit A (amoA) genes revealed that the bacterial homologue predominated by at 
      least three orders of magnitude in all samples. Archaeal amoA was only detectable 
      in approximately 15% of these samples. Using terminal restriction fragment length 
      polymorphism analysis, we monitored AOB lineages based on amoA genes. The 
      Nitrosomonas europaea lineage and a novel Nitrosomonas-like cluster were the 
      dominant AOB signatures, with a Nitrosospira lineage present at lower relative 
      abundance. These lineages exhibited strong temporal oscillations, with one 
      becoming sequentially dominant over the other. Using non-metric multidimensional 
      scaling and redundancy analyses, we tested correlations between terminal 
      restriction fragment length polymorphism profiles and 20 operational and 
      environmental parameters. The redundancy analyses indicated that the dynamics of 
      AOB lineages correlated most strongly with temperature, dissolved oxygen and 
      influent nitrite and chromium. The Nitrosospira lineage signal had a strong 
      negative correlation to dissolved oxygen and temperature, while the 
      Nitrosomonas-like (negative correlations) and N. europaea lineages (positive 
      correlations) were inversely linked (relative to one another) to influent nitrite 
      and chromium. Overall, this study suggests that AOA may be minor contributors to 
      ammonia oxidation in highly aerated activated sludge, and provides insight into 
      parameters controlling the diversity and dominance of AOB lineages within 
      bioreactors during periods of stable nitrification.
FAU - Wells, George F
AU  - Wells GF
AD  - Environmental Engineering and Science Program, Stanford University, Stanford, CA 
      94305, USA.
FAU - Park, Hee-Deung
AU  - Park HD
FAU - Yeung, Chok-Hang
AU  - Yeung CH
FAU - Eggleston, Brad
AU  - Eggleston B
FAU - Francis, Christopher A
AU  - Francis CA
FAU - Criddle, Craig S
AU  - Criddle CS
LA  - eng
SI  - GENBANK/DQ444189
SI  - GENBANK/DQ444190
SI  - GENBANK/DQ444191
SI  - GENBANK/DQ444192
SI  - GENBANK/DQ444193
SI  - GENBANK/DQ444194
SI  - GENBANK/DQ444195
SI  - GENBANK/DQ444196
SI  - GENBANK/DQ444197
SI  - GENBANK/DQ444198
SI  - GENBANK/DQ444199
SI  - GENBANK/DQ444200
SI  - GENBANK/DQ444201
SI  - GENBANK/DQ444202
SI  - GENBANK/DQ444203
SI  - GENBANK/DQ444204
SI  - GENBANK/DQ444205
SI  - GENBANK/DQ444206
SI  - GENBANK/DQ444207
SI  - GENBANK/DQ444208
SI  - GENBANK/DQ444209
SI  - GENBANK/DQ444210
SI  - GENBANK/DQ444211
SI  - GENBANK/DQ444212
SI  - GENBANK/DQ444213
SI  - GENBANK/DQ444214
SI  - GENBANK/DQ444215
SI  - GENBANK/DQ444216
SI  - GENBANK/DQ444217
SI  - GENBANK/DQ444218
SI  - GENBANK/DQ682408
SI  - GENBANK/DQ682409
SI  - GENBANK/DQ682410
SI  - GENBANK/DQ682411
SI  - GENBANK/DQ682412
SI  - GENBANK/DQ682413
SI  - GENBANK/DQ682414
SI  - GENBANK/DQ682415
SI  - GENBANK/DQ682416
SI  - GENBANK/DQ682417
SI  - GENBANK/DQ682418
SI  - GENBANK/DQ682419
SI  - GENBANK/DQ682420
SI  - GENBANK/DQ682421
SI  - GENBANK/DQ682422
SI  - GENBANK/DQ682423
SI  - GENBANK/DQ682424
SI  - GENBANK/DQ682425
SI  - GENBANK/DQ682426
SI  - GENBANK/DQ682427
SI  - GENBANK/DQ682428
SI  - GENBANK/DQ682429
SI  - GENBANK/DQ682430
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090604
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Sewage)
RN  - 7664-41-7 (Ammonia)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Archaea/growth & development/metabolism
MH  - Base Sequence
MH  - Betaproteobacteria/classification/growth & development/*metabolism
MH  - Bioreactors/*microbiology
MH  - Genes, Bacterial
MH  - Molecular Sequence Data
MH  - Nitrogen/metabolism
MH  - Phylogeny
MH  - Sewage/*chemistry
MH  - Waste Disposal, Fluid
EDAT- 2009/06/12 09:00
MHDA- 2009/10/22 06:00
CRDT- 2009/06/12 09:00
PHST- 2009/06/12 09:00 [entrez]
PHST- 2009/06/12 09:00 [pubmed]
PHST- 2009/10/22 06:00 [medline]
AID - EMI1958 [pii]
AID - 10.1111/j.1462-2920.2009.01958.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2009 Sep;11(9):2310-28. doi: 10.1111/j.1462-2920.2009.01958.x. 
      Epub 2009 Jun 4.