PMID- 19515986
OWN - NLM
STAT- MEDLINE
DCOM- 20090818
LR  - 20200930
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 297
IP  - 2
DP  - 2009 Aug
TI  - Role of purinergic P2Y1 receptors in regulation of vasopressin and oxytocin 
      secretion.
PG  - R478-84
LID - 10.1152/ajpregu.00163.2009 [doi]
AB  - Pharmacological studies demonstrated that ATP elevates intracellular calcium 
      ([Ca(2+)](i)) in supraoptic nucleus (SON) neurons primarily by activation of P2X2 
      and P2Y1 purinergic receptors [P2Y1R]. The current studies provide evidence for 
      the presence of P2Y1R protein in SON neurons, evidence that activation of these 
      P2Y1Rs induces an increase in [Ca(2+)](i) from both intracellular stores and 
      Ca(2+) influx, and functional evidence that activation of P2Y1Rs induces 
      vasopressin (VP) and oxytocin (OT) hormone release. Pretreatment of Fura-2 
      AM-loaded explants of the hypothalamo-neurohypophyseal system (HNS) with 
      thapsigargin (TG) significantly (approximately 80%) reduced the increase in 
      [Ca(2+)](i) induced by the P2Y1R-specific agonist, 2-methylthio-ADP (2-MeSADP). 
      In contrast, the increase in [Ca(2+)](i) was slightly (approximately 20%) 
      decreased in calcium-free medium. The calcium response to 2-MeSADP was completely 
      blocked by the P2Y1R-specific antagonist, MRS2179 or by a combination of TG 
      pretreatment and calcium-free medium. It was absent in P2Y1R knockout mice 
      (P2Y1R(-/-)). 2-MeSADP significantly increased VP and OT release from perifused 
      rat and wild-type mouse HNS explants compared with control. MRS2179 prevented 
      this response in wild-type mouse, but it did not prevent ATP-induced hormone 
      release from rat explants. 2-MeSADP did not induce hormone release from 
      P2Y1R(-/-) explants. These findings support a potential role for P2Y1Rs in 
      regulation of VP and OT release. The finding that P2Y1R activation induces a 
      small Ca(2+) influx suggests that P2Y1Rs may regulate VP release by modifying ion 
      channels such as stretch-inactivated cation channels.
FAU - Song, Zhilin
AU  - Song Z
AD  - Dept. of Physiology and Biophysics, School of Medicine, Univ. of Colorado Denver, 
      Denver, CO, USA. zhilin.song@ucdenver.edu
FAU - Gomes, Dayane A
AU  - Gomes DA
FAU - Stevens, Wanida
AU  - Stevens W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090610
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (N(6)-methyl-2'-deoxyadenosine 3',5'-diphosphate)
RN  - 0 (P2ry1 protein, mouse)
RN  - 0 (P2ry1 protein, rat)
RN  - 0 (Purinergic P2 Receptor Agonists)
RN  - 0 (Purinergic P2 Receptor Antagonists)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2Y1)
RN  - 0 (Thionucleotides)
RN  - 11000-17-2 (Vasopressins)
RN  - 34983-48-7 (methylthio-ADP)
RN  - 50-56-6 (Oxytocin)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Diphosphate/analogs & derivatives/pharmacology
MH  - Adenosine Triphosphate/pharmacology
MH  - Animals
MH  - Calcium Signaling/drug effects
MH  - Hypothalamo-Hypophyseal System/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neurons/metabolism
MH  - Oxytocin/*metabolism
MH  - Paraventricular Hypothalamic Nucleus/metabolism
MH  - Perfusion
MH  - Purinergic P2 Receptor Agonists
MH  - Purinergic P2 Receptor Antagonists
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2/*physiology
MH  - Receptors, Purinergic P2Y1
MH  - Supraoptic Nucleus/*metabolism
MH  - Thapsigargin/pharmacology
MH  - Thionucleotides/pharmacology
MH  - Vasopressins/*metabolism
EDAT- 2009/06/12 09:00
MHDA- 2009/08/19 09:00
CRDT- 2009/06/12 09:00
PHST- 2009/06/12 09:00 [entrez]
PHST- 2009/06/12 09:00 [pubmed]
PHST- 2009/08/19 09:00 [medline]
AID - 00163.2009 [pii]
AID - 10.1152/ajpregu.00163.2009 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2009 Aug;297(2):R478-84. doi: 
      10.1152/ajpregu.00163.2009. Epub 2009 Jun 10.