PMID- 1951684
OWN - NLM
STAT- MEDLINE
DCOM- 19911220
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 261
IP  - 5 Pt 1
DP  - 1991 Nov
TI  - Fasting inhibits insulin-mediated glycolysis and anaplerosis in human skeletal 
      muscle.
PG  - E598-605
AB  - Euglycemic (approximately 5.5 mM) hyperinsulinemic (60 mU.m-2.min-1) clamps were 
      performed for 2 h after a 10-h fast and after a prolonged (72-h) fast. Biopsies 
      were obtained from the quadriceps femoris muscle before and after each clamp. The 
      rate of whole body glucose disposal was approximately 50% lower during the clamp 
      after the 72-h fast (P less than or equal to 0.001). The increase in carbohydrate 
      (CHO) oxidation (which is proportional to glycolysis) during the clamp after the 
      10-h fast (to 13.8 +/- 1.5 mumol.kg fat free mass-1.min-1) was completely 
      abolished during the clamp after the 72-h fast (1.7 +/- 0.6; P less than or equal 
      to 0.001). During the clamp after the 10-h fast, postphosphofructokinase (PFK) 
      intermediates and malate in muscle increased, whereas glutamate decreased (P less 
      than or equal to 0.05-0.001 vs. basal) and citrate did not change. During the 
      clamp after the 72-h fast, there were no significant changes in post-PFK 
      intermediates or glutamate (P greater than 0.05 vs. basal), but there was a 
      decrease in citrate (P less than or equal to 0.01 vs. basal). Euglycemic 
      hyperinsulinemia increased glycogen synthase fractional activity in muscle under 
      both conditions but to a greater extent after the 72-h fast (P less than or equal 
      to 0.01). It is concluded that insulin (after 10-h fast) increases glycolytic 
      flux and the content of malate in muscle, which is probably due to increased 
      anaplerosis.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Castillo, C E
AU  - Castillo CE
AD  - Clinical Diabetes and Nutrition Section, National Institute of Diabetes, 
      Digestive, and Kidney Diseases, National Institutes of Health, Phoenix, Arizona 
      85106.
FAU - Katz, A
AU  - Katz A
FAU - Spencer, M K
AU  - Spencer MK
FAU - Yan, Z
AU  - Yan Z
FAU - Nyomba, B L
AU  - Nyomba BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Insulin)
RN  - EC 2.4.1.11 (Glycogen Synthase)
RN  - EC 2.7.- (Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Body Composition
MH  - Carbohydrate Metabolism
MH  - *Fasting
MH  - Glucose/biosynthesis
MH  - Glycogen Synthase/metabolism
MH  - *Glycolysis
MH  - Humans
MH  - Insulin/pharmacology/*physiology
MH  - Male
MH  - Muscles/*metabolism
MH  - Oxidation-Reduction
MH  - Protein Kinases/metabolism
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
AID - 10.1152/ajpendo.1991.261.5.E598 [doi]
PST - ppublish
SO  - Am J Physiol. 1991 Nov;261(5 Pt 1):E598-605. doi: 
      10.1152/ajpendo.1991.261.5.E598.