PMID- 19517998
OWN - NLM
STAT- MEDLINE
DCOM- 20091009
LR  - 20131121
IS  - 0301-1208 (Print)
IS  - 0301-1208 (Linking)
VI  - 46
IP  - 2
DP  - 2009 Apr
TI  - Interactions of bacterial polysaccharides with cationic dyes: physicochemical 
      studies.
PG  - 192-7
AB  - Capsular polysaccharides (SPS) are an integral component of gram-negative 
      bacteria, and also have potential use as vaccine. In this paper, interactions of 
      SPS isolated from Klebsiella strains K20 and K51 with cationic dyes pinacyanol 
      chloride (PCYN) and acridine orange (AO) were studied by absorbance and 
      fluorescence measurements. Both the polysaccharides having glucuronic acid as the 
      potential anionic site induced strong metachromasy (blue shift approximately 100 
      nm) in the PCYN. The spectral changes were studied at different polymer/dye molar 
      ratios (P/D = 0-40). A complete reversal of metachromasy was observed upon 
      addition of co-solvents, suggesting the breakaway of dye molecules from the 
      biopolymer matrix. Binding constant, changes in free energy, enthalpy and entropy 
      of the dye polymer complex were also computed from the spectral data at different 
      temperatures to reveal the nature of the interaction. Quenching of fluorescence 
      of AO by the polymers and the incorporated mechanisms were also explored.
FAU - Dasgupta, S
AU  - Dasgupta S
AD  - Department of Chemistry, Tripura University, Suryamaninagar, Tripura 799 130, 
      India.
FAU - Nath, R K
AU  - Nath RK
FAU - Biswas, S
AU  - Biswas S
FAU - Mitra, A
AU  - Mitra A
FAU - Panda, A K
AU  - Panda AK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Biochem Biophys
JT  - Indian journal of biochemistry & biophysics
JID - 0310774
RN  - 0 (Carbocyanines)
RN  - 0 (Coloring Agents)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 3K9958V90M (Ethanol)
RN  - 91SZ6DGY86 (quinaldine blue)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Absorption/drug effects
MH  - Acridine Orange/*metabolism
MH  - Carbocyanines/*metabolism
MH  - Coloring Agents/*metabolism
MH  - Ethanol/pharmacology
MH  - Klebsiella/*chemistry
MH  - Polysaccharides, Bacterial/isolation & purification/*metabolism
MH  - Spectrum Analysis
MH  - Temperature
MH  - Thermodynamics
EDAT- 2009/06/13 09:00
MHDA- 2009/10/10 06:00
CRDT- 2009/06/13 09:00
PHST- 2009/06/13 09:00 [entrez]
PHST- 2009/06/13 09:00 [pubmed]
PHST- 2009/10/10 06:00 [medline]
PST - ppublish
SO  - Indian J Biochem Biophys. 2009 Apr;46(2):192-7.