PMID- 19522772
OWN - NLM
STAT- MEDLINE
DCOM- 20090821
LR  - 20191210
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 74
IP  - 1
DP  - 2009 Jul
TI  - High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class I 
      ligands using real-time PCR.
PG  - 73-80
LID - 10.1111/j.1399-0039.2009.01265.x [doi]
AB  - Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are 
      critical components of innate immunity. Interactions between KIRs and their human 
      leukocyte antigen (HLA) ligands have been shown to influence autoimmune and 
      infectious disease course in defined populations. However, the low throughput and 
      high cost of current methods impede confirmation of the universality of these 
      findings. To support large epidemiology surveys, we developed a high-throughput 
      real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, 
      KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 
      100% sensitivity and specificity in detection of carrier and non-carrier on 
      reference samples. The application of this platform will further clarify the 
      nature and impact of the KIR-HLA epistatic interaction on disease course in large 
      global population-based studies.
FAU - Koehler, R N
AU  - Koehler RN
AD  - Division of Retrovirology, US Military HIV Research Program/Henry M. Jackson 
      Foundation, Rockville, MD 20850, USA. rkoehler@hivresearch.org
FAU - Walsh, A M
AU  - Walsh AM
FAU - Moqueet, N
AU  - Moqueet N
FAU - Currier, J R
AU  - Currier JR
FAU - Eller, M A
AU  - Eller MA
FAU - Eller, L A
AU  - Eller LA
FAU - Wabwire-Mangen, F
AU  - Wabwire-Mangen F
FAU - Michael, N L
AU  - Michael NL
FAU - Robb, M L
AU  - Robb ML
FAU - McCutchan, F E
AU  - McCutchan FE
FAU - Kijak, G H
AU  - Kijak GH
LA  - eng
GR  - Y1-A1-2642-11/PHS HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (KIR2DL2 protein, human)
RN  - 0 (KIR2DL3 protein, human)
RN  - 0 (KIR3DL1 protein, human)
RN  - 0 (Ligands)
RN  - 0 (Receptors, KIR2DL2)
RN  - 0 (Receptors, KIR2DL3)
RN  - 0 (Receptors, KIR3DL1)
RN  - 0 (Receptors, KIR3DS1)
SB  - IM
MH  - Alleles
MH  - Genotype
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Ligands
MH  - Polymerase Chain Reaction/*methods
MH  - Receptors, KIR2DL2/*genetics
MH  - Receptors, KIR2DL3/*genetics
MH  - Receptors, KIR3DL1/*genetics
MH  - Receptors, KIR3DS1/*genetics
MH  - Sensitivity and Specificity
EDAT- 2009/06/16 09:00
MHDA- 2009/08/22 09:00
CRDT- 2009/06/16 09:00
PHST- 2009/06/16 09:00 [entrez]
PHST- 2009/06/16 09:00 [pubmed]
PHST- 2009/08/22 09:00 [medline]
AID - TAN1265 [pii]
AID - 10.1111/j.1399-0039.2009.01265.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2009 Jul;74(1):73-80. doi: 10.1111/j.1399-0039.2009.01265.x.