PMID- 19523947
OWN - NLM
STAT- MEDLINE
DCOM- 20100127
LR  - 20211020
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 89
IP  - 4
DP  - 2009 Oct
TI  - Early keratocyte apoptosis after epithelial scrape injury in the human cornea.
PG  - 597-9
LID - 10.1016/j.exer.2009.06.003 [doi]
AB  - Animal studies in mice, rats, rabbits, pigs and hens demonstrated that anterior 
      keratocytes undergo programmed cell death or apoptosis after corneal epithelial 
      injury. Many other wound healing changes subsequently follow the keratocyte 
      apoptosis response. This study evaluated early keratocyte apoptosis after corneal 
      epithelial scrape injury in human eyes scheduled for enucleation for malignancy. 
      Two eyes had corneal epithelial scrape 1 h prior to the enucleation and another 
      eye served as a control and had no corneal scrape prior to enucleation. One 
      additional eye was enucleated, washed with balanced salt solution, and then had 
      the corneal epithelium scraped 1 h prior to processing for analysis. Apoptosis 
      was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) assay and confirmed by transmission electron microscopy (TEM). 
      Anterior keratocyte apoptosis was detected in the three corneas that had 
      epithelial scrape injury, but not in the control unwounded cornea. This study 
      confirmed that keratocyte apoptosis is also an early response to corneal 
      epithelial injury in humans and showed that tears are not essential for 
      keratocyte apoptosis to occur in response to epithelial injury.
FAU - Ambrosio, Renato Jr
AU  - Ambrosio R Jr
FAU - Kara-Jose, Newton
AU  - Kara-Jose N
FAU - Wilson, Steven E
AU  - Wilson SE
LA  - eng
GR  - R01 EY010056-14/EY/NEI NIH HHS/United States
GR  - EY010056/EY/NEI NIH HHS/United States
GR  - EY015638/EY/NEI NIH HHS/United States
GR  - R24 EY015638/EY/NEI NIH HHS/United States
GR  - R01 EY010056/EY/NEI NIH HHS/United States
PT  - Letter
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090611
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
SB  - IM
MH  - Adult
MH  - *Apoptosis
MH  - Cornea/*ultrastructure
MH  - Epithelial Cells/pathology
MH  - Epithelium, Corneal/*injuries
MH  - Eye Diseases/surgery
MH  - Eye Enucleation
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Middle Aged
MH  - Wound Healing
MH  - Wounds and Injuries/*pathology
PMC - PMC2743797
MID - NIHMS123792
EDAT- 2009/06/16 09:00
MHDA- 2010/01/28 06:00
PMCR- 2010/10/01
CRDT- 2009/06/16 09:00
PHST- 2009/04/14 00:00 [received]
PHST- 2009/05/21 00:00 [revised]
PHST- 2009/06/01 00:00 [accepted]
PHST- 2009/06/16 09:00 [entrez]
PHST- 2009/06/16 09:00 [pubmed]
PHST- 2010/01/28 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - S0014-4835(09)00155-9 [pii]
AID - 10.1016/j.exer.2009.06.003 [doi]
PST - ppublish
SO  - Exp Eye Res. 2009 Oct;89(4):597-9. doi: 10.1016/j.exer.2009.06.003. Epub 2009 Jun 
      11.