PMID- 19524419
OWN - NLM
STAT- MEDLINE
DCOM- 20091214
LR  - 20240109
IS  - 1618-095X (Electronic)
IS  - 0944-7113 (Linking)
VI  - 16
IP  - 10
DP  - 2009 Oct
TI  - Budlein A from Viguiera robusta inhibits leukocyte-endothelial cell interactions, 
      adhesion molecule expression and inflammatory mediators release.
PG  - 904-15
LID - 10.1016/j.phymed.2009.04.002 [doi]
AB  - Budlein A has been reported to exert some analgesic and anti-inflammatory 
      properties. In this study, we have evaluated its effect on LPS-induced leukocyte 
      recruitment in vivo and the mechanisms involved in its anti-inflammatory 
      activity. In vivo, intravital videomicroscopy was used to determine the effects 
      of budlein A on LPS-induced leukocyte-endothelial cell interactions in the murine 
      cremasteric microcirculation. In vitro, the effects of budlein A on LPS-induced 
      cytokine, chemokine and nitrites release, T-cell proliferative response as well 
      as cell adhesion molecule expression (CAM) were evaluated. In vivo, 
      intraperitoneal administration of budlein A (2.6 mM/kg) caused a significant 
      reduction of LPS-induced leukocyte rolling flux, adhesion and emigration by 84, 
      92 and 96% respectively. In vitro, T-cell proliferative response was also 
      affected by budlein A. When murine J774 macrophages were incubated with the 
      sesquiterpene lactone, LPS-induced IL-1beta, tumor necrosis factor-alpha 
      (TNF-alpha) and keratinocyte-derived chemokine (KC) release were 
      concentration-dependently inhibited. In human umbilical vein endothelial cells 
      (HUVECs), budlein A also reduced the production of TNF-alpha, monocyte 
      chemoattractant protein-1 (MCP-1), IL-8, nitrites and CAM expression elicited by 
      LPS. Budlein A is a potent inhibitor of LPS-induced leukocyte accumulation in 
      vivo. This effect appears to be mediated through inhibition of cytokine and 
      chemokine release and down-regulation of CAM expression. Thus, it has potential 
      therapeutic interest for the control of leukocyte recruitment that occurs in 
      different inflammatory disorders.
FAU - Nicolete, Roberto
AU  - Nicolete R
AD  - Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av. do Cafe 
      s/n, 14040-903 Ribeirao Preto, Brazil.
FAU - Arakawa, Nilton S
AU  - Arakawa NS
FAU - Rius, Cristina
AU  - Rius C
FAU - Nomizo, Auro
AU  - Nomizo A
FAU - Jose, Peter J
AU  - Jose PJ
FAU - Da Costa, Fernando B
AU  - Da Costa FB
FAU - Sanz, Maria-Jesus
AU  - Sanz MJ
FAU - Faccioli, Lucia H
AU  - Faccioli LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090612
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokines)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Lactones)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nitrites)
RN  - 0 (Plant Extracts)
RN  - 0 (Sesquiterpenes)
RN  - 0 (budlein A)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Asteraceae/*chemistry
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Communication/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Chemokines/metabolism
MH  - Dexamethasone
MH  - Endothelial Cells/*drug effects
MH  - Humans
MH  - Immunosuppressive Agents/*isolation & purification
MH  - Lactones/isolation & purification/*pharmacology
MH  - Leukocyte Rolling/*drug effects
MH  - Lipopolysaccharides
MH  - Macrophages/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Microcirculation
MH  - Nitric Oxide/metabolism
MH  - Nitrites/metabolism
MH  - Plant Extracts/pharmacology
MH  - Sesquiterpenes/isolation & purification/*pharmacology
MH  - T-Lymphocytes/drug effects
EDAT- 2009/06/16 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/06/16 09:00
PHST- 2009/01/31 00:00 [received]
PHST- 2009/03/20 00:00 [revised]
PHST- 2009/04/21 00:00 [accepted]
PHST- 2009/06/16 09:00 [entrez]
PHST- 2009/06/16 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0944-7113(09)00106-8 [pii]
AID - 10.1016/j.phymed.2009.04.002 [doi]
PST - ppublish
SO  - Phytomedicine. 2009 Oct;16(10):904-15. doi: 10.1016/j.phymed.2009.04.002. Epub 
      2009 Jun 12.