PMID- 19524871
OWN - NLM
STAT- MEDLINE
DCOM- 20091001
LR  - 20141120
IS  - 1931-5244 (Print)
IS  - 1878-1810 (Linking)
VI  - 154
IP  - 1
DP  - 2009 Jul
TI  - Stimulation of CCL2 (MCP-1) and CCL2 mRNA by substance P in LAD2 human mast 
      cells.
PG  - 27-33
LID - 10.1016/j.trsl.2009.03.006 [doi]
AB  - Chemokines are cytokines with chemotactic properties on inflammatory cells and 
      other cell types. Chemokine (C-C motif) ligand 2 (CCL2), which is also called 
      monocyte chemotactic protein 1 (MCP-1), is a potent chemotactic molecule that 
      attracts lymphocytes, monocytes, mast cells, and memory T cells, but not 
      neutrophils. CCL2/MCP-1 represents a link between the activation of monocytes, 
      lymphocytes, basophils, mast cells, and eosinophils in inflammatory disorders, 
      such as the late-phase allergic reaction. This C-C chemokine also plays a role in 
      regulating Th-cell cytokine production and leukocyte trafficking. Laboratory of 
      allergic diseases (LAD) cells is the first reported human mast cell line that 
      closely resembles a primary culture of CD34+-derived human mast cells. These 
      cells were cultured in vitro and treated with different concentrations of 
      substance P (SP) for the production of CCL2/MCP-1. We used calcium ionophore as a 
      positive control for stimulating transcription and translation of CCL2/MCP-1. The 
      stimulation of SP on CCL2/MCP-1 was statistically significant (P < 0.05) compared 
      with the control (untreated cells). In this study, we determined the expression 
      and secretion of CCL2/MCP-1 from SP-activated LAD2 human mast cells in vitro. The 
      levels of CCL2/MCP-1 from SP-activated LAD2 human mast cells were higher at 10 
      microM and at 18 h incubation compared with controls. This effect was also 
      revealed on CCL2/MCP-1 messenger RNA (mRNA) expression, as determined by reverse 
      transcriptase polymerase chain reaction (RT-PCR) analysis. Our data suggest that 
      SP is an important neurotransmitter that can stimulate the chemokine CCL2, which 
      plays a fundamental role in inflammation by recruiting inflammatory cells to 
      specific cites.
FAU - Castellani, Maria Luisa
AU  - Castellani ML
AD  - Immunology Division, Medical School, University of Chieti-Pescara, Chieti, Italy.
FAU - Vecchiet, Jacopo
AU  - Vecchiet J
FAU - Salini, Vincenzo
AU  - Salini V
FAU - Conti, Pio
AU  - Conti P
FAU - Theoharides, Theoharis C
AU  - Theoharides TC
FAU - Caraffa, Auro
AU  - Caraffa A
FAU - Antinolfi, Pierluigi
AU  - Antinolfi P
FAU - Tete, Stefano
AU  - Tete S
FAU - Ciampoli, Cristian
AU  - Ciampoli C
FAU - Cuccurullo, Chiara
AU  - Cuccurullo C
FAU - Cerulli, Giuliano
AU  - Cerulli G
FAU - Felaco, Mario
AU  - Felaco M
FAU - Boscolo, Paolo
AU  - Boscolo P
LA  - eng
PT  - Journal Article
DEP - 20090422
PL  - United States
TA  - Transl Res
JT  - Translational research : the journal of laboratory and clinical medicine
JID - 101280339
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 33507-63-0 (Substance P)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/*genetics
MH  - Chemotaxis, Leukocyte/drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - In Vitro Techniques
MH  - Mast Cells/*drug effects/*physiology
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Substance P/*pharmacology
EDAT- 2009/06/16 09:00
MHDA- 2009/10/02 06:00
CRDT- 2009/06/16 09:00
PHST- 2008/09/22 00:00 [received]
PHST- 2009/03/22 00:00 [revised]
PHST- 2009/03/23 00:00 [accepted]
PHST- 2009/06/16 09:00 [entrez]
PHST- 2009/06/16 09:00 [pubmed]
PHST- 2009/10/02 06:00 [medline]
AID - S1931-5244(09)00083-8 [pii]
AID - 10.1016/j.trsl.2009.03.006 [doi]
PST - ppublish
SO  - Transl Res. 2009 Jul;154(1):27-33. doi: 10.1016/j.trsl.2009.03.006. Epub 2009 Apr 
      22.