PMID- 19526522
OWN - NLM
STAT- MEDLINE
DCOM- 20100203
LR  - 20131121
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 72
IP  - 11
DP  - 2009 Nov
TI  - Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein 
      expression in the testis of prepubertal rats.
PG  - 868-77
LID - 10.1002/jemt.20741 [doi]
AB  - The aim of this study was to analyze whether di(2-ethylhexyl) phthalate (DEHP), a 
      Sertoli and Leydig cell toxicant, is able to induce alterations in the expression 
      of testicular gap and tight junction proteins. DEHP was administered by gavage (1 
      g/5 mL corn oil/kg body weight/day) to 25-day-old male Sprague-Dawley rats for 2 
      days (DEHP-27d) and control rats were treated with corn-oil vehicle for 2 days 
      (C-27d); animals were killed 24 h after the last treatment. Testes of DEHP-27d 
      rats showed different degrees of germ cell sloughing of seminiferous tubules 
      (ST). No alterations of the blood testis barrier (BTB) by lanthanum tracer study 
      were observed. ST of DEHP-27d rats showed a milder immunofluorescence and more 
      restricted expression of connexin-43 (Cx43) in the adluminal and basal 
      compartment compared to C-27d. In DEHP-27d rats, we found a discontinuous 
      immunofluorescent (IF) pattern for zonula occludens (ZO-1), contrasting with the 
      continuous IF profile observed in C-27d, and a delocalization of claudin-11. A 
      decrease in Cx43 and ZO-1 and no changes in occludin expression were detected by 
      Western blot in the testes of DEHP-27d rats. Results from 57-day-old rats treated 
      with DEHP for 2 days and held for 30 days without treatment showed that the 
      alterations in protein expression induced by DEHP are reversible. However, a 
      delay of spermatogenesis compared to C-57d rats, occurred. Data demonstrated that 
      DEHP does not impair BTB permeability but induces germ cell sloughing that might 
      respond to a down regulation of Cx43 and ZO-1 that alters cell junction proteins.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Sobarzo, Cristian M
AU  - Sobarzo CM
AD  - Instituto de Investigaciones en Reproduccion, Facultad de Medicina Universidad de 
      Buenos Aires, Paraguay 2155 P10, C 1121 ABG, Buenos Aires, Argentina. 
      ciruba@fmed.uba.ar
FAU - Lustig, Livia
AU  - Lustig L
FAU - Ponzio, Roberto
AU  - Ponzio R
FAU - Suescun, Maria Olga
AU  - Suescun MO
FAU - Denduchis, Berta
AU  - Denduchis B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Connexins)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
SB  - IM
MH  - Animals
MH  - Connexins/*biosynthesis
MH  - Diethylhexyl Phthalate/*toxicity
MH  - Gap Junctions/*drug effects
MH  - Leydig Cells/drug effects
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/*drug effects
MH  - Sertoli Cells/drug effects
MH  - Testis/*drug effects
MH  - Tight Junctions/*drug effects
EDAT- 2009/06/16 09:00
MHDA- 2010/02/04 06:00
CRDT- 2009/06/16 09:00
PHST- 2009/06/16 09:00 [entrez]
PHST- 2009/06/16 09:00 [pubmed]
PHST- 2010/02/04 06:00 [medline]
AID - 10.1002/jemt.20741 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2009 Nov;72(11):868-77. doi: 10.1002/jemt.20741.