PMID- 19527167
OWN - NLM
STAT- MEDLINE
DCOM- 20090925
LR  - 20090702
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 200
IP  - 3
DP  - 2009 Aug 1
TI  - Aspergillus fumigatus induces immunoglobulin E-independent mast cell 
      degranulation.
PG  - 464-72
LID - 10.1086/600070 [doi]
AB  - BACKGROUND: Pulmonary colonization by Aspergillus fumigatus in chronic lung 
      disease is associated with progressive decline in lung function even in the 
      absence of specific allergic response. We hypothesized that A. fumigatus 
      contributes to this decline by inducing pulmonary mast cell degranulation even in 
      the absence of antigen-specific immunoglobulin E (IgE). Therefore, we 
      investigated whether A. fumigatus can induce mast cell degranulation 
      independently of IgE. METHODS: We studied the interactions of Aspergillus species 
      with mast cells in the absence of IgE in vitro with use of scanning electron 
      microscopy. The extent of mast cell degranulation was quantified by measuring the 
      release of beta-hexosaminidase. RESULTS: Mature A. fumigatus hyphae induced mast 
      cell degranulation in the absence of IgE. Hyphae of Aspergillus flavus, 
      Aspergillus niger, and Aspergillus nidulans induced much less mast cell 
      degranulation. Mast cell degranulation required direct contact with mature A. 
      fumigatus hyphae, and was not induced by conidia or immature hyphae. Killed 
      hyphae induced significant degranulation, whereas live hyphae from mutants 
      deficient in the fungal development regulators StuA and MedA induced very little 
      degranulation. CONCLUSIONS: Factors expressed on the surface of mature A. 
      fumigatus hyphae that are controlled by StuA and MedA induce mast cell 
      degranulation in the absence of IgE.
FAU - Urb, Mirjam
AU  - Urb M
AD  - Department of Microbiology, McGill University, Montreal, Quebec, Canada.
FAU - Pouliot, Philippe
AU  - Pouliot P
FAU - Gravelat, Fabrice N
AU  - Gravelat FN
FAU - Olivier, Martin
AU  - Olivier M
FAU - Sheppard, Donald C
AU  - Sheppard DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 67-99-2 (Gliotoxin)
SB  - IM
MH  - Animals
MH  - Aspergillus fumigatus/*physiology
MH  - Cell Adhesion
MH  - *Cell Degranulation
MH  - Cells, Cultured
MH  - Gliotoxin/pharmacology
MH  - Hyphae
MH  - Immunoglobulin E/*metabolism
MH  - Mast Cells/drug effects/*microbiology/*physiology
MH  - Rats
EDAT- 2009/06/17 09:00
MHDA- 2009/09/26 06:00
CRDT- 2009/06/17 09:00
PHST- 2009/06/17 09:00 [entrez]
PHST- 2009/06/17 09:00 [pubmed]
PHST- 2009/09/26 06:00 [medline]
AID - 10.1086/600070 [doi]
PST - ppublish
SO  - J Infect Dis. 2009 Aug 1;200(3):464-72. doi: 10.1086/600070.