PMID- 19527452
OWN - NLM
STAT- MEDLINE
DCOM- 20090803
LR  - 20090616
IS  - 1365-3024 (Electronic)
IS  - 0141-9838 (Linking)
VI  - 31
IP  - 7
DP  - 2009 Jul
TI  - Neospora caninum and bone marrow-derived dendritic cells: parasite survival, 
      proliferation, and induction of cytokine expression.
PG  - 366-72
LID - 10.1111/j.1365-3024.2009.01112.x [doi]
AB  - Dendritic cells (DCs) represent the first line defence of the innate immune 
      system following infection with pathogens. We exploratively addressed invasion 
      and survival ability of Neospora caninum, a parasite causing abortion in cattle, 
      in mouse bone marrow DCs (BMDCs), and respective cytokine expression patterns. 
      Immature BMDCs were exposed to viable (untreated) and nonviable parasites that 
      had been inactivated by different means. Invasion and/or internalization, as well 
      as intracellular survival and proliferation of tachyzoites were determined by 
      NcGRA2-RT-PCR and transmission electron microscopy (TEM). Cytokine expression was 
      evaluated by reverse transcription (RT)-PCR and cytokine ELISA. Transmission 
      electron microscopy of DCs stimulated with untreated viable parasites revealed 
      that N. caninum was able to invade and proliferate within BMDCs. This was 
      confirmed by NcGRA2-RT-PCR. On the other hand, no viable parasite organisms were 
      revealed by TEM when exposing BMDCs to inactivated parasites (nonviability 
      demonstrated by NcGRA2-RT-PCR). Cytokine expression analysis (as assessed by both 
      RT-PCR and ELISA) demonstrated that both viable and nonviable parasites 
      stimulated mBMDCs to express IL-12p40, IL-10 and TNF-alpha, whereas IL-4 RNA 
      expression was not detected. Thus, exposure of mBMDCs to both viable and 
      nonviable parasites results in the expression of cytokines that are relevant for 
      a mixed Th1/Th2 immune response.
FAU - Strohbusch, M
AU  - Strohbusch M
AD  - Institute of Parasitology, University of Berne, Bern, Switzerland.
FAU - Muller, N
AU  - Muller N
FAU - Hemphill, A
AU  - Hemphill A
FAU - Margos, M
AU  - Margos M
FAU - Grandgirard, D
AU  - Grandgirard D
FAU - Leib, S
AU  - Leib S
FAU - Greif, G
AU  - Greif G
FAU - Gottstein, B
AU  - Gottstein B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Parasite Immunol
JT  - Parasite immunology
JID - 7910948
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis
MH  - Cytosol/parasitology
MH  - Dendritic Cells/*immunology/*parasitology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Microbial Viability
MH  - Microscopy, Electron, Transmission/methods
MH  - Neospora/*growth & development/*immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
EDAT- 2009/06/17 09:00
MHDA- 2009/08/04 09:00
CRDT- 2009/06/17 09:00
PHST- 2009/06/17 09:00 [entrez]
PHST- 2009/06/17 09:00 [pubmed]
PHST- 2009/08/04 09:00 [medline]
AID - PIM1112 [pii]
AID - 10.1111/j.1365-3024.2009.01112.x [doi]
PST - ppublish
SO  - Parasite Immunol. 2009 Jul;31(7):366-72. doi: 10.1111/j.1365-3024.2009.01112.x.