PMID- 19528150
OWN - NLM
STAT- MEDLINE
DCOM- 20091102
LR  - 20220310
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 58
IP  - Pt 10
DP  - 2009 Oct
TI  - Bacterial colonization of enamel in situ investigated using fluorescence in situ 
      hybridization.
PG  - 1359-1366
LID - 10.1099/jmm.0.011213-0 [doi]
AB  - Oral biofilms are one of the greatest challenges in dental research. The present 
      study aimed to investigate initial bacterial colonization of enamel surfaces in 
      situ using fluorescence in situ hybridization (FISH) over a 12 h period. For this 
      purpose, bovine enamel slabs were fixed on buccal sites of individual splints 
      worn by six subjects for 2, 6 and 12 h to allow biofilm formation. Specimens were 
      processed for FISH and evaluated with confocal laser-scanning microscopy, using 
      probes for eubacteria, Streptococcus species, Veillonella species, Fusobacterium 
      nucleatum and Actinomyces naeslundii. The number of adherent bacteria increased 
      with time and all tested bacterial species were detected in the biofilm formed in 
      situ. The general percentage composition of the eubacteria did not change over 
      the investigated period, but the number of streptococci, the most frequently 
      detected species, increased significantly with time (2 h: 17.7+/-13.8 %; 6 h: 
      20.0+/-16.6 %; 12 h: 24.7+/-16.1 %). However, < or =1 % of the surface was 
      covered with bacteria after 12 h of biofilm formation in situ. In conclusion, 
      FISH is an appropriate method for quantifying initial biofilm formation in situ, 
      and the proportion of streptococci increases during the first 12 h of bacterial 
      adherence.
FAU - Al-Ahmad, Ali
AU  - Al-Ahmad A
AD  - Department of Operative Dentistry and Periodontology, University of Freiburg, 
      Hugstetter Strasse 55, D-79106 Freiburg, Germany.
FAU - Follo, Marie
AU  - Follo M
AD  - Department of Hematology and Oncology, Core Facility, Albert-Ludwig University, 
      D-79085 Freiburg, Germany.
FAU - Selzer, Ann-Carina
AU  - Selzer AC
AD  - Department of Operative Dentistry and Periodontology, University of Freiburg, 
      Hugstetter Strasse 55, D-79106 Freiburg, Germany.
FAU - Hellwig, Elmar
AU  - Hellwig E
AD  - Department of Operative Dentistry and Periodontology, University of Freiburg, 
      Hugstetter Strasse 55, D-79106 Freiburg, Germany.
FAU - Hannig, Matthias
AU  - Hannig M
AD  - Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, 
      University Hospital, Saarland University, Building 73, D-66421 Homburg/Saar, 
      Germany.
FAU - Hannig, Christian
AU  - Hannig C
AD  - Department of Operative Dentistry and Periodontology, University of Freiburg, 
      Hugstetter Strasse 55, D-79106 Freiburg, Germany.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090615
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Actinomyces/genetics/isolation & purification
MH  - Animals
MH  - Bacteria/genetics/isolation & purification/ultrastructure
MH  - *Bacterial Adhesion/genetics
MH  - Base Sequence
MH  - Biofilms/growth & development
MH  - Cattle
MH  - DNA, Bacterial/genetics
MH  - Dental Enamel/*microbiology/ultrastructure
MH  - Fusobacterium nucleatum/genetics/isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Streptococcus/genetics/isolation & purification
MH  - Veillonella/genetics/isolation & purification
EDAT- 2009/06/17 09:00
MHDA- 2009/11/03 06:00
CRDT- 2009/06/17 09:00
PHST- 2009/06/17 09:00 [entrez]
PHST- 2009/06/17 09:00 [pubmed]
PHST- 2009/11/03 06:00 [medline]
AID - 10.1099/jmm.0.011213-0 [doi]
PST - ppublish
SO  - J Med Microbiol. 2009 Oct;58(Pt 10):1359-1366. doi: 10.1099/jmm.0.011213-0. Epub 
      2009 Jun 15.