PMID- 19531354
OWN - NLM
STAT- MEDLINE
DCOM- 20090714
LR  - 20131121
IS  - 1878-1551 (Electronic)
IS  - 1534-5807 (Linking)
VI  - 16
IP  - 6
DP  - 2009 Jun
TI  - Methylation of the sterol nucleus by STRM-1 regulates dauer larva formation in 
      Caenorhabditis elegans.
PG  - 833-43
LID - 10.1016/j.devcel.2009.04.012 [doi]
AB  - In response to pheromone(s), Caenorhabditis elegans interrupts its reproductive 
      life cycle and enters diapause as a stress-resistant dauer larva. This decision 
      is governed by a complex system of neuronal and hormonal regulation. All the 
      signals converge onto the nuclear hormone receptor DAF-12. A sterol-derived 
      hormone, dafachronic acid (DA), supports reproductive development by binding to 
      DAF-12 and inhibiting its dauer-promoting activity. Here, we identify a 
      methyltransferase, STRM-1, that modulates DA levels and thus dauer formation. By 
      modifying the substrates that are used for the synthesis of DA, STRM-1 can reduce 
      the amount of hormone produced. Loss of STRM-1 function leads to elevated levels 
      of DA and inefficient dauer formation. Sterol methylation was not previously 
      recognized as a mechanism for regulating hormone activity. Moreover, the C-4 
      sterol nucleus methylation catalyzed by STRM-1 is unique to nematodes and thus 
      could be a target for therapeutic strategies against parasitic nematode 
      infections.
FAU - Hannich, J Thomas
AU  - Hannich JT
AD  - Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
FAU - Entchev, Eugeni V
AU  - Entchev EV
FAU - Mende, Fanny
AU  - Mende F
FAU - Boytchev, Hristio
AU  - Boytchev H
FAU - Martin, Rene
AU  - Martin R
FAU - Zagoriy, Vyacheslav
AU  - Zagoriy V
FAU - Theumer, Gabriele
AU  - Theumer G
FAU - Riezman, Isabelle
AU  - Riezman I
FAU - Riezman, Howard
AU  - Riezman H
FAU - Knolker, Hans-Joachim
AU  - Knolker HJ
FAU - Kurzchalia, Teymuras V
AU  - Kurzchalia TV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Cell
JT  - Developmental cell
JID - 101120028
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Cholestenes)
RN  - 0 (DAF-12 protein, C elegans)
RN  - 0 (Pheromones)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Sterols)
RN  - 0 (dafachronic acid)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.- (DAF-9 protein, C elegans)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.- (Protein Methyltransferases)
RN  - EC 2.1.1.- (STRM-1 protein, C elegans)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/cytology/enzymology/*growth & development/*metabolism
MH  - Caenorhabditis elegans Proteins/genetics/*metabolism
MH  - Cholestenes/metabolism
MH  - Cholesterol/metabolism
MH  - Cytochrome P-450 Enzyme System/metabolism
MH  - Gene Deletion
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Larva/cytology/drug effects/growth & development/metabolism
MH  - Methylation/drug effects
MH  - Methyltransferases/genetics/*metabolism
MH  - Models, Biological
MH  - Pheromones/pharmacology
MH  - Protein Methyltransferases/metabolism
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Sterols/chemistry/*metabolism
MH  - Substrate Specificity/drug effects
EDAT- 2009/06/18 09:00
MHDA- 2009/07/15 09:00
CRDT- 2009/06/18 09:00
PHST- 2008/07/13 00:00 [received]
PHST- 2009/02/24 00:00 [revised]
PHST- 2009/04/24 00:00 [accepted]
PHST- 2009/06/18 09:00 [entrez]
PHST- 2009/06/18 09:00 [pubmed]
PHST- 2009/07/15 09:00 [medline]
AID - S1534-5807(09)00174-9 [pii]
AID - 10.1016/j.devcel.2009.04.012 [doi]
PST - ppublish
SO  - Dev Cell. 2009 Jun;16(6):833-43. doi: 10.1016/j.devcel.2009.04.012.