PMID- 19533012
OWN - NLM
STAT- MEDLINE
DCOM- 20091217
LR  - 20090714
IS  - 1432-0827 (Electronic)
IS  - 0171-967X (Linking)
VI  - 85
IP  - 1
DP  - 2009 Jul
TI  - Hyperhomocysteinemia is associated with impaired fracture healing in mice.
PG  - 17-21
LID - 10.1007/s00223-009-9262-6 [doi]
AB  - Hyperhomocysteinemia (HHCY) has been shown to disturb bone metabolism and to 
      increase the incidence of osteoporosis and osteoporotic fractures. However, there 
      is a complete lack of information on whether these metabolic alterations affect 
      bone repair. The aim of this study was to analyze the impact of HHCY on fracture 
      healing. One group of mice was fed a homocystine-supplemented diet (n = 12), 
      whereas another group received the accordant standard diet for control (n = 13). 
      Four weeks after the stable fixation of a closed femoral fracture, animals were 
      killed to prepare bones for histomorphometric and biomechanical analyses. In 
      addition, blood samples were obtained to evaluate serum concentration of 
      homocysteine (HCY). Quantitative analysis of blood samples revealed severe HHCY 
      as indicated by significantly increased serum concentrations of HCY in animals 
      fed the homocystine-supplemented diet (102.2 +/- 64.5 micromol/l) compared to 
      controls (2.8 +/- 1.5 micromol/l). Biomechanical evaluation of bone repair 
      revealed significantly decreased bending stiffness of the femora of 
      homocystine-fed animals (45.5 +/- 18.2 N/mm) compared with controls (64.6 +/- 
      15.8 N/mm). Histomorphometric analysis demonstrated a slightly smaller callus 
      diameter in HHCY animals but no significant differences in the tissue composition 
      of the callus. In conclusion, the homocystine-supplemented diet leads to severe 
      HHCY, which is associated with an impaired biomechanical quality of the healing 
      bone.
FAU - Claes, L
AU  - Claes L
AD  - Institute of Orthopedic Research and Biomechanics, University of Ulm, 
      Helmholtzstrasse 14, Ulm 89081, Germany. lutz.claes@uni-ulm.de
FAU - Schmalenbach, J
AU  - Schmalenbach J
FAU - Herrmann, M
AU  - Herrmann M
FAU - Olku, I
AU  - Olku I
FAU - Garcia, P
AU  - Garcia P
FAU - Histing, T
AU  - Histing T
FAU - Obeid, R
AU  - Obeid R
FAU - Schorr, H
AU  - Schorr H
FAU - Herrmann, W
AU  - Herrmann W
FAU - Pohlemann, T
AU  - Pohlemann T
FAU - Menger, M D
AU  - Menger MD
FAU - Holstein, J H
AU  - Holstein JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090617
PL  - United States
TA  - Calcif Tissue Int
JT  - Calcified tissue international
JID - 7905481
SB  - IM
MH  - Animals
MH  - Bone Density/physiology
MH  - Disease Models, Animal
MH  - Femoral Fractures/*etiology
MH  - Femur/metabolism/*pathology
MH  - *Fracture Healing
MH  - Hyperhomocysteinemia/*complications/metabolism
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Osteoporosis/metabolism
EDAT- 2009/06/18 09:00
MHDA- 2009/12/18 06:00
CRDT- 2009/06/18 09:00
PHST- 2008/11/21 00:00 [received]
PHST- 2009/05/12 00:00 [accepted]
PHST- 2009/06/18 09:00 [entrez]
PHST- 2009/06/18 09:00 [pubmed]
PHST- 2009/12/18 06:00 [medline]
AID - 10.1007/s00223-009-9262-6 [doi]
PST - ppublish
SO  - Calcif Tissue Int. 2009 Jul;85(1):17-21. doi: 10.1007/s00223-009-9262-6. Epub 
      2009 Jun 17.