PMID- 19534393
OWN - NLM
STAT- MEDLINE
DCOM- 20090804
LR  - 20160510
IS  - 1210-7875 (Print)
IS  - 1210-7875 (Linking)
VI  - 45
IP  - 2
DP  - 2009 Apr
TI  - [Our experience in using fluorescence in situ hybridization FISH-Uro Vysion in 
      diagnostics of urothelial carcinoma].
PG  - 46-9
AB  - Urothelial carcinoma is a disease at high risk of recurrence after the initial 
      therapy (70-80%) and with the tendency to progression accomplishing the 
      recurrence (30%). Long lasting monitoring of patients with urothelial carcinoma 
      is necessary. Cystoscopy and cytology are currently the primary modalities used 
      to detect and monitor urothelial carcinoma. However, cytology has relatively poor 
      sensitivity especially in well differentiated tumors. Cystoscopy is an invasive 
      and relatively expensive method. Therefore, methods improving detection of 
      urothelial carcinoma from urine specimens are employed. Uro Vysion (Vysis) 
      fluorescence in situ hybridization (FISH) for improved detection of urothelial 
      carcinoma was evaluated. MATERIALS AND METHODS: Bladder tumor progression is 
      accompanied by increased chromosomal instability and aneuploidy of chromosomes 3, 
      7, 17 and loss of locus 9p21. A total of 124 patients were analyzed at Dpts. of 
      Urology and Pathology, Faculty Hospital in Brno. Cytologically analyzed urine 
      specimens were tested by FISH and simultaneously cystoscopy was employed 
      including biopsy for histological examination. RESULTS: FISH analysis was 
      positive in 35 cases, including 5 cases with negative biopsy and cytology. 
      Negative FISH result was detected in 24 cases where the malignant status was 
      determined. The sensitivity of FISH in our series was 58.9% and the specificity 
      88.1%. CONCLUSIONS: FISH is a relatively simple, speedy and non invasive 
      diagnostic method. It detects the symptoms of malignity on the molecular level, 
      which leads to earlier diagnosis and therapy and, hence, to potential extended 
      survival. FISH makes it possible to take decision in cases of atypical or unclear 
      cytological finding. The FISH method using the Uro Vysion kit appears as a 
      prospective non invasive method capable of early UK detection, with a higher 
      sensitivity than the standard cytology of urine.
FAU - Vit, V
AU  - Vit V
AD  - Urologicka klinika FN a LF MU, Brno. v-vit@email.cz
FAU - Pacik, D
AU  - Pacik D
FAU - Cermak, A
AU  - Cermak A
FAU - Falkova, I
AU  - Falkova I
FAU - Smardova, J
AU  - Smardova J
FAU - Hrabalkova, R
AU  - Hrabalkova R
FAU - Svitakova, M
AU  - Svitakova M
FAU - Pavlovsky, Z
AU  - Pavlovsky Z
FAU - Votava, M
AU  - Votava M
LA  - cze
PT  - English Abstract
PT  - Journal Article
TT  - Nase zkusenosti s metodou fluorescencni in situ hybridizace pri detekci 
      urotelialniho karcinomu.
PL  - Czech Republic
TA  - Cesk Patol
JT  - Ceskoslovenska patologie
JID - 0050734
SB  - IM
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Sensitivity and Specificity
MH  - Urinary Bladder Neoplasms/*diagnosis
EDAT- 2009/06/19 09:00
MHDA- 2009/08/06 09:00
CRDT- 2009/06/19 09:00
PHST- 2009/06/19 09:00 [entrez]
PHST- 2009/06/19 09:00 [pubmed]
PHST- 2009/08/06 09:00 [medline]
PST - ppublish
SO  - Cesk Patol. 2009 Apr;45(2):46-9.