PMID- 19536878
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20110214
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 117
IP  - 4
DP  - 2009 Aug 25
TI  - Cytogenetics and fluorescence in situ hybridization as adjuncts to cytology in 
      the diagnosis of malignant mesothelioma.
PG  - 247-53
LID - 10.1002/cncy.20036 [doi]
AB  - BACKGROUND: Virtually all malignant mesotheliomas (MMs) exhibit clonal 
      chromosomal aberrations. Although the chromosome regions affected by these 
      aberration(s) may vary from 1 tumor to another, certain regions are commonly 
      disrupted. These aberrations are absent in benign mesothelial cells, and 
      therefore their presence can be used to confirm a diagnosis of MM. In the current 
      study, the authors investigated the value of karyotyping and fluorescence in situ 
      hybridization (FISH) as adjuncts to conventional cytologic examination in 
      patients with MM. METHODS: A retrospective analysis of 48 pleural or peritoneal 
      fluids from patients with histologically confirmed MM was performed. Karyotypic 
      analyses were attempted in all cases. In 27 cases, FISH for deletions of 9p21 
      (CDKN2A gene) and 22q was also performed because the karyotype was normal or 
      unsuccessful. RESULTS: Karyotypes were obtained in 35 (73%) of the specimens. Of 
      these, 15 (43%) were abnormal and 20 (57%) were normal. Thirteen additional 
      abnormal results were detected by FISH in cases for which the karyotypes were 
      normal or unsuccessful. A total of 24 cases (50%) had an associated cytologic 
      interpretation. Karyotyping or FISH was abnormal in 8 cases that were interpreted 
      cytologically as either negative or suspicious. CONCLUSIONS: The combination of 
      FISH and karyotyping was found to improve on the diagnostic sensitivity of 
      karyotyping alone in detecting MM in effusions. The authors concluded that 
      karyotyping and FISH together were a more useful adjunct to cytology than FISH or 
      karyotyping alone.
CI  - Copyright 2009 American Cancer Society
FAU - Factor, Rachel E
AU  - Factor RE
AD  - Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, 
      Boston, Massachusetts, USA. rachel.factor@bmc.org
FAU - Dal Cin, Paola
AU  - Dal Cin P
FAU - Fletcher, Jonathan A
AU  - Fletcher JA
FAU - Cibas, Edmund S
AU  - Cibas ES
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Cytogenetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Mesothelioma/diagnosis/*genetics
MH  - Middle Aged
EDAT- 2009/06/19 09:00
MHDA- 2009/09/09 06:00
CRDT- 2009/06/19 09:00
PHST- 2009/06/19 09:00 [entrez]
PHST- 2009/06/19 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
AID - 10.1002/cncy.20036 [doi]
PST - ppublish
SO  - Cancer. 2009 Aug 25;117(4):247-53. doi: 10.1002/cncy.20036.