PMID- 1953745
OWN - NLM
STAT- MEDLINE
DCOM- 19911202
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 180
IP  - 2
DP  - 1991 Oct 31
TI  - Electrochemical identification of dopachrome isomerase in Drosophila 
      melanogaster.
PG  - 724-9
AB  - A principal reaction in the eumelanin biosynthetic pathway is the conversion of 
      dopachrome (DC) to dihydroxyindole(s). Dopachrome isomerase (DI), the enzyme that 
      catalyzes this reaction, was detected for the first time in larvae of D. 
      melanogaster. Unlike the enzyme from B16 mouse melanoma cells which converts 
      dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), the insect enzyme 
      forms 5,6-dihydroxyindole (DHI). The activity of the insect DI was linear through 
      15 min incubation, and the amount of DHI produced was proportional to the amount 
      of enzyme that was incorporated into the reaction mixtures.
FAU - Li, J
AU  - Li J
AD  - Department of Biology, Loyola University of Chicago, IL 60626.
FAU - Nappi, A J
AU  - Nappi AJ
LA  - eng
GR  - AI 24199/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Indolequinones)
RN  - 0 (Indoles)
RN  - 0 (Quinones)
RN  - 3571-34-4 (dopachrome)
SB  - IM
MH  - Animals
MH  - Drosophila melanogaster/*enzymology
MH  - Electrochemistry/methods
MH  - *Indolequinones
MH  - Indoles/chemical synthesis
MH  - Kinetics
MH  - Larva
MH  - Melanoma, Experimental/enzymology
MH  - Mice
MH  - Quinones/chemical synthesis
EDAT- 1991/10/31 00:00
MHDA- 1991/10/31 00:01
CRDT- 1991/10/31 00:00
PHST- 1991/10/31 00:00 [pubmed]
PHST- 1991/10/31 00:01 [medline]
PHST- 1991/10/31 00:00 [entrez]
AID - S0006-291X(05)81125-8 [pii]
AID - 10.1016/s0006-291x(05)81125-8 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1991 Oct 31;180(2):724-9. doi: 
      10.1016/s0006-291x(05)81125-8.