PMID- 1953794
OWN - NLM
STAT- MEDLINE
DCOM- 19911216
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 180
IP  - 3
DP  - 1991 Nov 14
TI  - The thapsigargin-sensitive intracellular Ca2+ pool is more important in plasma 
      membrane Ca2+ entry than the IP3-sensitive intracellular Ca2+ pool in neuronal 
      cell lines.
PG  - 1518-26
AB  - In NG108-15 cells, bradykinin (BK) and thapsigargin (TG) caused transient 
      increases in a cytosolic free Ca2+ concentration ([Ca2+]i), after which [Ca2+]i 
      elevated by TG only declined to a higher, sustained level than an unstimulated 
      level. In PC12 cells, carbachol (CCh) evoked a transient increase in [Ca2+]i 
      followed by a sustained rise of [Ca2+]i, whereas [Ca2+]i elevated by TG almost 
      maintained its higher level. In the absence of extracellular Ca2+, the sustained 
      elevation of [Ca2+]i induced by each drug we used was abolished. In addition, the 
      rise in [Ca2+]i stimulated by TG was less affected after CCh or BK, whereas CCh 
      or BK caused no increase in [Ca2+]i after TG. TG neither increased cellular 
      inositol phosphates nor modified the inositol phosphates format on stimulated by 
      CCh or BK. We conclude that TG may release Ca2+ from both IP3-sensitive and 
      -insensitive intracellular pools and that some kinds of signalling to link the 
      intracellular Ca2+ pools and Ca2+ entry seem to exist in neuronal cells.
FAU - Takemura, H
AU  - Takemura H
AD  - Department of Pharmacology, Sapporo Medical College, Japan.
FAU - Ohshika, H
AU  - Ohshika H
FAU - Yokosawa, N
AU  - Yokosawa N
FAU - Oguma, K
AU  - Oguma K
FAU - Thastrup, O
AU  - Thastrup O
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Inositol Phosphates)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (Terpenes)
RN  - 0 (omega-Conotoxins)
RN  - 105344-37-4 (fura-2-am)
RN  - 107407-86-3 (Conus magus toxin)
RN  - 57WA9QZ5WH (Nimodipine)
RN  - 67526-95-8 (Thapsigargin)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - S8TIM42R2W (Bradykinin)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Bradykinin/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Cell Line
MH  - Cell Membrane/drug effects/metabolism
MH  - Fluorescent Dyes
MH  - Fura-2/analogs & derivatives
MH  - Glioma
MH  - Inositol 1,4,5-Trisphosphate/*metabolism
MH  - Inositol Phosphates/*metabolism
MH  - Kinetics
MH  - Neuroblastoma
MH  - Nimodipine/pharmacology
MH  - PC12 Cells
MH  - Peptides, Cyclic/pharmacology
MH  - Terpenes/*pharmacology
MH  - Thapsigargin
MH  - *omega-Conotoxins
EDAT- 1991/11/14 00:00
MHDA- 1991/11/14 00:01
CRDT- 1991/11/14 00:00
PHST- 1991/11/14 00:00 [pubmed]
PHST- 1991/11/14 00:01 [medline]
PHST- 1991/11/14 00:00 [entrez]
AID - S0006-291X(05)81368-3 [pii]
AID - 10.1016/s0006-291x(05)81368-3 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1991 Nov 14;180(3):1518-26. doi: 
      10.1016/s0006-291x(05)81368-3.