PMID- 19538522
OWN - NLM
STAT- MEDLINE
DCOM- 20110209
LR  - 20101102
IS  - 1439-0507 (Electronic)
IS  - 0933-7407 (Linking)
VI  - 53
IP  - 6
DP  - 2010 Nov
TI  - Biotype stability of Candida albicans isolates after culture storage determined 
      by randomly amplified polymorphic DNA and phenotypical methods.
PG  - 468-74
LID - 10.1111/j.1439-0507.2009.01741.x [doi]
AB  - Typing methods to evaluate isolates in relation to their phenotypical and 
      molecular characteristics are essential in epidemiological studies. In this 
      study, Candida albicans biotypes were determined before and after storage in 
      order to verify their stability. Twenty C. albicans isolates were typed by 
      Randomly Amplified Polymorphic DNA (RAPD), production of phospholipase and 
      proteinase exoenzymes (enzymotyping) and morphotyping before and after 180 days 
      of storage in Sabouraud dextrose agar (SDA) and sterilised distilled water. 
      Before the storage, 19 RAPD patterns, two enzymotypes and eight morphotypes were 
      identified. The fragment patterns obtained by RAPD, on the one hand, were not 
      significantly altered after storage. On the other hand, the majority of the 
      isolates changed their enzymotype and morphotype after storage. RAPD typing 
      provided the better discriminatory index (DI) among isolates (DI = 0.995) and 
      maintained the profile identified, thereby confirming its utility in 
      epidemiological surveys. Based on the low reproducibility observed after storage 
      in SDA and distilled water by morphotyping (DI = 0.853) and enzymotyping (DI = 
      0.521), the use of these techniques is not recommended on stored isolates.
CI  - (c) 2009 Blackwell Verlag GmbH.
FAU - Bacelo, Katia Leston
AU  - Bacelo KL
AD  - Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, 
      Ribeirao Preto, Sao Paulo, Brazil. katialbacelo@gmail.com
FAU - da Costa, Karen Regina Carim
AU  - da Costa KR
FAU - Ferreira, Joseane Cristina
AU  - Ferreira JC
FAU - Candido, Regina Celia
AU  - Candido RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mycoses
JT  - Mycoses
JID - 8805008
RN  - 0 (DNA, Fungal)
RN  - 0 (Fungal Proteins)
RN  - EC 3.1.- (Phospholipases)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Candida albicans/classification/*genetics/*metabolism
MH  - DNA, Fungal/genetics
MH  - Fungal Proteins/metabolism
MH  - Genomic Instability
MH  - Genotype
MH  - Humans
MH  - Mycological Typing Techniques
MH  - Mycology/*methods
MH  - Peptide Hydrolases/metabolism
MH  - Phenotype
MH  - Phospholipases/metabolism
MH  - *Preservation, Biological
MH  - Random Amplified Polymorphic DNA Technique
MH  - Specimen Handling/*methods
EDAT- 2009/06/23 09:00
MHDA- 2011/02/10 06:00
CRDT- 2009/06/23 09:00
PHST- 2009/06/23 09:00 [entrez]
PHST- 2009/06/23 09:00 [pubmed]
PHST- 2011/02/10 06:00 [medline]
AID - MYC1741 [pii]
AID - 10.1111/j.1439-0507.2009.01741.x [doi]
PST - ppublish
SO  - Mycoses. 2010 Nov;53(6):468-74. doi: 10.1111/j.1439-0507.2009.01741.x.