PMID- 19538931
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20211020
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 393
IP  - 1
DP  - 2009 Oct 1
TI  - Quantification of carnosine-related peptides by microchip electrophoresis with 
      chemiluminescence detection.
PG  - 105-10
LID - 10.1016/j.ab.2009.06.012 [doi]
AB  - A microchip electrophoresis (MCE) method with chemiluminescence (CL) detection 
      was developed for the determination of carnosine-related peptides, including 
      carnosine, homocarnosine, and anserine, in biological samples. A simple 
      integrated MCE-CL system was built to perform the assays. The highly sensitive CL 
      detection was achieved by means of the CL reaction between hydrogen peroxide and 
      N-(4-aminobutyl)-N-ethylisoluminol-tagged peptides in the presence of adenine as 
      a CL enhancer and Co(2+) as a catalyst. Experimental conditions for analyte 
      labeling, MCE separation, and CL detection were studied. MCE separation of the 
      above-mentioned three peptides took less than 120 s. Detection limits 
      (signal/noise ratio [S/N]=3) of 3.0x10(-8), 2.8x10(-8), and 3.4x10(-8) M were 
      obtained for carnosine, anserine, and homocarnosine, respectively. The current 
      MCE-CL method was applied for the determination of carnosine, anserine, and 
      homocarnosine in human cerebrospinal fluid (CSF) and canine plasma. Homocarnosine 
      was detected at the micromolar (microM) level in the CSF samples analyzed, 
      whereas the levels of carnosine and anserine in these samples were below the 
      detection limit of the assay. Interestingly, both carnosine and anserine were 
      detected in the canine plasma samples, whereas homocarnosine was not.
FAU - Zhao, Shulin
AU  - Zhao S
AD  - College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 
      51004, People's Republic of China.
FAU - Huang, Yong
AU  - Huang Y
FAU - Shi, Ming
AU  - Shi M
FAU - Huang, Junming
AU  - Huang J
FAU - Liu, Yi-Ming
AU  - Liu YM
LA  - eng
GR  - S06 GM008047/GM/NIGMS NIH HHS/United States
GR  - S06 GM008047-360044/GM/NIGMS NIH HHS/United States
GR  - S06GM08047/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090616
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 5EXP385Q4F (Luminol)
RN  - 66612-29-1 (6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione)
RN  - 8HO6PVN24W (Carnosine)
SB  - IM
MH  - Animals
MH  - Carnosine/*analysis
MH  - Cerebrospinal Fluid/chemistry
MH  - Dogs
MH  - Electrophoresis, Microchip/*methods
MH  - Humans
MH  - Luminescent Measurements/*methods
MH  - Luminol/analogs & derivatives
MH  - Reproducibility of Results
PMC - PMC2744379
MID - NIHMS132739
EDAT- 2009/06/23 09:00
MHDA- 2009/10/01 06:00
PMCR- 2010/10/01
CRDT- 2009/06/23 09:00
PHST- 2009/04/18 00:00 [received]
PHST- 2009/06/10 00:00 [revised]
PHST- 2009/06/11 00:00 [accepted]
PHST- 2009/06/23 09:00 [entrez]
PHST- 2009/06/23 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - S0003-2697(09)00409-6 [pii]
AID - 10.1016/j.ab.2009.06.012 [doi]
PST - ppublish
SO  - Anal Biochem. 2009 Oct 1;393(1):105-10. doi: 10.1016/j.ab.2009.06.012. Epub 2009 
      Jun 16.