PMID- 19539716
OWN - NLM
STAT- MEDLINE
DCOM- 20090923
LR  - 20211203
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 461
IP  - 2
DP  - 2009 Sep 18
TI  - Hydrogen peroxide induces autophagic cell death in C6 glioma cells via 
      BNIP3-mediated suppression of the mTOR pathway.
PG  - 131-5
LID - 10.1016/j.neulet.2009.06.011 [doi]
AB  - Oxidative stress by exposure to H(2)O(2) induces various types of cell death 
      depending on cell type and conditions. We report herein on a study of the 
      mechanisms underlying H(2)O(2)-induced cell death in C6 glioma cells. The 
      findings show that H(2)O(2) triggers a caspase-independent autophagic cell death 
      in these cells. The findings also show that H(2)O(2) induces the 
      dephosphorylation of the mammalian target of rapamycin (mTOR) at Ser 2481 and the 
      p70 ribosomal protein S6 kinase (p70S6K) at Thr389 in a Bcl-2/E1B 19kDa 
      interacting protein 3 (BNIP3)-dependent manner. BNIP3 has the capacity to inhibit 
      mTOR activity and mTOR inhibition plays a role in autophagic induction. This 
      suggests that BNIP3 may mediate H(2)O(2)-induced autophagic cell death through 
      the suppression of mTOR. The findings show that the down-regulation of BNIP3 by 
      BNIP3 siRNA prevents C6 cells from undergoing H(2)O(2)-induced autophagic cell 
      death. Collectively, these results suggest that H(2)O(2) induces autophagic cell 
      death in C6 cells via the BNIP3-mediated suppression of the mTOR pathway.
FAU - Byun, Yu Jeong
AU  - Byun YJ
AD  - Department of Biochemistry, College of Medicine, The Catholic University of 
      Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Republic of Korea.
FAU - Kim, Seong Keun
AU  - Kim SK
FAU - Kim, Young Mi
AU  - Kim YM
FAU - Chae, Gue Tae
AU  - Chae GT
FAU - Jeong, Seong-Whan
AU  - Jeong SW
FAU - Lee, Seong-Beom
AU  - Lee SB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090617
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (BNIP3 protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *Autophagy
MH  - Cell Line, Tumor
MH  - Glioma
MH  - Hydrogen Peroxide/*pharmacology
MH  - Membrane Proteins/*physiology
MH  - Mitochondrial Proteins
MH  - *Oxidative Stress
MH  - Phosphorylation
MH  - Protein Kinases/*physiology
MH  - Proto-Oncogene Proteins/*physiology
MH  - Rats
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases
EDAT- 2009/06/23 09:00
MHDA- 2009/09/24 06:00
CRDT- 2009/06/23 09:00
PHST- 2009/04/14 00:00 [received]
PHST- 2009/05/21 00:00 [revised]
PHST- 2009/06/10 00:00 [accepted]
PHST- 2009/06/23 09:00 [entrez]
PHST- 2009/06/23 09:00 [pubmed]
PHST- 2009/09/24 06:00 [medline]
AID - S0304-3940(09)00807-6 [pii]
AID - 10.1016/j.neulet.2009.06.011 [doi]
PST - ppublish
SO  - Neurosci Lett. 2009 Sep 18;461(2):131-5. doi: 10.1016/j.neulet.2009.06.011. Epub 
      2009 Jun 17.