PMID- 19539975
OWN - NLM
STAT- MEDLINE
DCOM- 20091019
LR  - 20131121
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 43
IP  - 14
DP  - 2009 Aug
TI  - Quantitative and qualitative analysis of methanogenic communities in 
      mesophilically and psychrophilically cultivated anaerobic granular biofilims.
PG  - 3365-74
LID - 10.1016/j.watres.2009.03.039 [doi]
AB  - Anaerobic granulation describes the self-immobilisation of methanogenic consortia 
      into dense, particulate biofilms. This procedure underpins the operation of 
      several categories of high-rate anaerobic wastewater treatment system. Full-scale 
      anaerobic granular sludge plants have been generally operated in the mesophilic 
      (20-45 degrees C) or thermophilic (45-65 degrees C) temperature range. On the 
      other hand, recent studies highlighted the economic advantages of treating 
      wastewaters at their discharge temperatures (mostly under 18 degrees C), removing 
      a costly heating process and increasing net biogas yield. However, as yet, 
      relatively little information is available about the microbial behaviour and 
      interactions in anaerobic granular sludge formed under psychrophilic conditions. 
      To this end, and in order to provide a microbial insight into low-temperature 
      anaerobic granulation, we monitored the changes in methanogenic community 
      structure, associated with the changes in process performance. Three, 
      laboratory-scale, expanded granular sludge bed (EGSB) bioreactors treating a 
      synthetic glucose wastewater were tested at two temperatures of 37+/-1 degrees C 
      (R1) and 15+/-1 degrees C (R2 and 3). Quantitative real-time PCR and specific 
      methanogenic activity assays highlighted a community shift towards 
      hydrogenotrophic methanogens, particularly the order Methanomicrobiales in the 
      low-temperature bioreactors. Corresponding to this, denaturing gradient gel 
      electrophoresis (DGGE) analysis identified the emergence and maintenance of a 
      Methanocorpusculum-like organism. Our results indicate that hydrogenotrophic 
      methanogens, particularly the Methanomicrobiales-related populations, are likely 
      to play important roles in low-temperature anaerobic granular sludge systems. 
      This suggests that the process efficiency could be improved by facilitating the 
      growth and retention of this group.
FAU - O'Reilly, Joe
AU  - O'Reilly J
AD  - Microbial Ecology Laboratory, Department of Microbiology and Environmental Change 
      Institute, National University of Ireland, Galway (NUI, Galway), University Road, 
      Galway, Ireland.
FAU - Lee, Changsoo
AU  - Lee C
FAU - Collins, Gavin
AU  - Collins G
FAU - Chinalia, Fabio
AU  - Chinalia F
FAU - Mahony, Therese
AU  - Mahony T
FAU - O'Flaherty, Vincent
AU  - O'Flaherty V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090405
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Anaerobiosis
MH  - Archaea/genetics/*growth & development
MH  - Biofilms/*growth & development
MH  - Bioreactors/microbiology
MH  - *Cold Temperature
MH  - Electrophoresis
MH  - Methane/*metabolism
MH  - Oxygen/isolation & purification
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2009/06/23 09:00
MHDA- 2009/10/20 06:00
CRDT- 2009/06/23 09:00
PHST- 2009/02/02 00:00 [received]
PHST- 2009/03/23 00:00 [revised]
PHST- 2009/03/24 00:00 [accepted]
PHST- 2009/06/23 09:00 [entrez]
PHST- 2009/06/23 09:00 [pubmed]
PHST- 2009/10/20 06:00 [medline]
AID - S0043-1354(09)00208-5 [pii]
AID - 10.1016/j.watres.2009.03.039 [doi]
PST - ppublish
SO  - Water Res. 2009 Aug;43(14):3365-74. doi: 10.1016/j.watres.2009.03.039. Epub 2009 
      Apr 5.