PMID- 19540430
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20141120
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Linking)
VI  - 30
IP  - 7
DP  - 2009 Jul
TI  - Central Neuropeptide S inhibits distal colonic transit through activation of 
      central Neuropeptide S receptor in mice.
PG  - 1313-7
LID - 10.1016/j.peptides.2009.03.012 [doi]
AB  - Neuropeptide S (NPS), the endogenous ligand of NPS receptor (NPSR), regulates 
      many biological functions, including arousal, anxiety, locomotion and food 
      intake. NPSR mRNA is expressed in several regions of central autonomic network 
      through which the brain controls visceromotor and other responses essential for 
      survival. However, the role of NPS/NPSR system in regulating gastrointestinal 
      motor is still unknown. Here, we studied the effects of NPS on distal colonic 
      transit in mice. Intracerebroventricular (i.c.v.) injection of NPS (1-1000 pmol) 
      inhibited fecal pellet output and bead expulsion in a dose-dependent manner. 
      However, intraperitoneal injection of NPS (1000 and 10000 pmol) did not affect 
      fecal pellet output and bead expulsion. In vitro, NPS (0.1-10 microM) also did 
      not modulate distal colonic contractions. Furthermore, i.c.v. co-administration 
      of [D-Val(5)]NPS, a pure and potent NPSR antagonist, dose-dependently antagonized 
      the inhibitory effects of NPS on fecal pellet output and bead expulsion. In 
      conclusion, our results firstly indicate that central NPS inhibits distal colonic 
      transit through the activation of central NPSR, which implicate that NPS/NPSR 
      system might be a new target to treat function disorder of distal colon.
FAU - Han, Ren-Wen
AU  - Han RW
AD  - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of 
      Basic Medical Sciences, Lanzhou University, Lanzhou 730000, PR China.
FAU - Chang, Min
AU  - Chang M
FAU - Peng, Ya-Li
AU  - Peng YL
FAU - Qiao, Lian-yong
AU  - Qiao LY
FAU - Yin, Xin-Qiang
AU  - Yin XQ
FAU - Li, Wei
AU  - Li W
FAU - Wang, Rui
AU  - Wang R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090326
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Neuropeptides)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (neuropeptide S, mouse)
SB  - IM
MH  - Animals
MH  - Colon/*drug effects/physiology
MH  - Gastrointestinal Motility/*drug effects
MH  - Gastrointestinal Transit/*drug effects
MH  - In Vitro Techniques
MH  - Injections, Intraperitoneal
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Neuropeptides/*pharmacology
MH  - Receptors, G-Protein-Coupled/antagonists & inhibitors/*metabolism
EDAT- 2009/06/23 09:00
MHDA- 2009/09/18 06:00
CRDT- 2009/06/23 09:00
PHST- 2009/02/20 00:00 [received]
PHST- 2009/03/17 00:00 [revised]
PHST- 2009/03/17 00:00 [accepted]
PHST- 2009/06/23 09:00 [entrez]
PHST- 2009/06/23 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
AID - S0196-9781(09)00120-X [pii]
AID - 10.1016/j.peptides.2009.03.012 [doi]
PST - ppublish
SO  - Peptides. 2009 Jul;30(7):1313-7. doi: 10.1016/j.peptides.2009.03.012. Epub 2009 
      Mar 26.