PMID- 19543826
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20211020
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 30
IP  - 1
DP  - 2010 Jan
TI  - MDMA (Ecstasy) decreases the number of neurons and stem cells in embryonic 
      cortical cultures.
PG  - 13-21
LID - 10.1007/s10571-009-9426-y [doi]
AB  - Ecstasy, 3,4-methylenedioxymetamphetamine (MDMA), is a recreational drug used 
      among adolescents, including young pregnant women. MDMA passes the placental 
      barrier and may therefore influence fetal development. The aim was to investigate 
      the direct effect of MDMA on cortical cells using dissociated CNS cortex of rat 
      embryos, E17. The primary culture was exposed to a single dose of MDMA and 
      collected 5 days later. MDMA caused a dramatic, dose-dependent (100 and 400 
      microM) decrease in nestin-positive stem cell density, as well as a significant 
      reduction (400 microM) in NeuN-positive cells. By qPCR, MDMA (200 microM) caused 
      a significant decrease in mRNA expression of the 5HT3 receptor, dopamine D(1) 
      receptor, and glutamate transporter EAAT2-1, as well as an increase in mRNA 
      levels of the NMDA NR1 receptor subunit and the 5HT(1A) receptor. In conclusion, 
      MDMA caused a marked reduction in stem cells and neurons in embryonic cortical 
      primary cell cultures, which was accompanied by changes in mRNA expression of 
      specific receptors and transporters for glutamatergic and monoaminergic 
      neurotransmitters.
FAU - Kindlundh-Hogberg, Anna M S
AU  - Kindlundh-Hogberg AM
AD  - Department of Neuroscience, Uppsala University, Uppsala, Sweden. 
      Anna.Kindlundh@neuro.uu.se
FAU - Pickering, Chris
AU  - Pickering C
FAU - Wicher, Grzegorz
AU  - Wicher G
FAU - Hober, David
AU  - Hober D
FAU - Schioth, Helgi B
AU  - Schioth HB
FAU - Fex Svenningsen, Asa
AU  - Fex Svenningsen A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090619
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.22.- (Caspase 3)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Caspase 3/metabolism
MH  - Cell Count
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology/*embryology
MH  - Embryo, Mammalian/*cytology
MH  - Gene Expression Regulation/drug effects
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Neurons/*cytology/drug effects/enzymology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/*cytology/drug effects/enzymology
EDAT- 2009/06/23 09:00
MHDA- 2010/04/14 06:00
CRDT- 2009/06/23 09:00
PHST- 2008/06/24 00:00 [received]
PHST- 2009/06/04 00:00 [accepted]
PHST- 2009/06/23 09:00 [entrez]
PHST- 2009/06/23 09:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - 10.1007/s10571-009-9426-y [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2010 Jan;30(1):13-21. doi: 10.1007/s10571-009-9426-y. Epub 
      2009 Jun 19.