PMID- 19545891
OWN - NLM
STAT- MEDLINE
DCOM- 20091012
LR  - 20090804
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 30
IP  - 28
DP  - 2009 Oct
TI  - Integrating polyurethane culture substrates into poly(dimethylsiloxane) 
      microdevices.
PG  - 5241-50
LID - 10.1016/j.biomaterials.2009.05.066 [doi]
AB  - Poly(dimethylsiloxane) (PDMS)-based microdevices have enabled rapid, 
      high-throughput assessment of cellular response to precisely controlled 
      microenvironmental stimuli, including chemical, matrix and mechanical factors. 
      However, the use of PDMS as a culture substrate precludes long-term culture and 
      may significantly impact cell response. Here we describe a method to integrate 
      polyurethane (PU), a well-studied and clinically relevant biomaterial, into the 
      PDMS multilayer microfabrication process, enabling the exploration of long-term 
      cellular response on alternative substrates in microdevices. To demonstrate the 
      utility of these hybrid microdevices for cell culture, we compared initial cell 
      adhesion, cell spreading, and maintenance of protein patterns on PU and PDMS 
      substrates. Initial cell adhesion and cell spreading after three days were 
      comparable between collagen-coated PDMS and PU substrates (with or without 
      collagen coating), but significantly lower on native PDMS substrates. However, 
      for longer culture durations (> or = 6 days), cell spreading and protein adhesion 
      on PU substrates was significantly better than that on PDMS substrates, and 
      comparable to that on tissue culture-treated polystyrene. Thus, the use of a 
      generic polyurethane substrate in microdevices enables longer-term cell culture 
      than is possible with PDMS substrates. More generally, this technique can improve 
      the impact and applicability of microdevice-based research by facilitating the 
      use of alternate, relevant biomaterials while maintaining the advantages of using 
      PDMS for microdevice fabrication.
FAU - Moraes, Christopher
AU  - Moraes C
AD  - Department of Mechanical and Industrial Engineering, University of Toronto, 5 
      King's College Road, Toronto, Ontario M5S 3G8, Canada.
FAU - Kagoma, Yoan K
AU  - Kagoma YK
FAU - Beca, Bogdan M
AU  - Beca BM
FAU - Tonelli-Zasarsky, Rachel L M
AU  - Tonelli-Zasarsky RL
FAU - Sun, Yu
AU  - Sun Y
FAU - Simmons, Craig A
AU  - Simmons CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090709
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Polyurethanes)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Aorta/cytology
MH  - *Cell Adhesion
MH  - Cell Culture Techniques/*instrumentation/methods
MH  - Cells, Cultured
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Equipment Design
MH  - Microfluidic Analytical Techniques/*instrumentation/methods
MH  - Polyurethanes/*chemistry
MH  - Proteins/*chemistry
MH  - Swine
EDAT- 2009/06/24 09:00
MHDA- 2009/10/13 06:00
CRDT- 2009/06/24 09:00
PHST- 2009/04/19 00:00 [received]
PHST- 2009/05/25 00:00 [accepted]
PHST- 2009/06/24 09:00 [entrez]
PHST- 2009/06/24 09:00 [pubmed]
PHST- 2009/10/13 06:00 [medline]
AID - S0142-9612(09)00595-X [pii]
AID - 10.1016/j.biomaterials.2009.05.066 [doi]
PST - ppublish
SO  - Biomaterials. 2009 Oct;30(28):5241-50. doi: 10.1016/j.biomaterials.2009.05.066. 
      Epub 2009 Jul 9.