PMID- 19549533
OWN - NLM
STAT- MEDLINE
DCOM- 20090928
LR  - 20211020
IS  - 1872-6216 (Electronic)
IS  - 0047-6374 (Print)
IS  - 0047-6374 (Linking)
VI  - 130
IP  - 8
DP  - 2009 Aug
TI  - Anti-oxidative and anti-inflammatory vasoprotective effects of caloric 
      restriction in aging: role of circulating factors and SIRT1.
PG  - 518-27
LID - 10.1016/j.mad.2009.06.004 [doi]
AB  - Endothelial dysfunction, oxidative stress and inflammation are associated with 
      vascular aging and promote the development of cardiovascular disease. Caloric 
      restriction (CR) mitigates conditions associated with aging, but its effects on 
      vascular dysfunction during aging remain poorly defined. To determine whether CR 
      exerts vasoprotective effects in aging, aortas of ad libitum (AL) fed young and 
      aged and CR-aged F344 rats were compared. Aging in AL-rats was associated with 
      impaired acetylcholine-induced relaxation, vascular oxidative stress and 
      increased NF-kappaB activity. Lifelong CR significantly improved endothelial 
      function, attenuated vascular ROS production, inhibited NF-kappaB activity and 
      down-regulated inflammatory genes. To elucidate the role of circulating factors 
      in mediation of the vasoprotective effects of CR, we determined whether sera 
      obtained from CR animals can confer anti-oxidant and anti-inflammatory effects in 
      cultured coronary arterial endothelial cells (CAECs), mimicking the effects of 
      CR. In CAECs cultured in the presence of AL serum TNFalpha elicited oxidative 
      stress, NF-kappaB activation and inflammatory gene expression. By contrast, 
      treatment of CAECs with CR serum attenuated TNFalpha-induced ROS generation and 
      prevented NF-kappaB activation and induction of inflammatory genes. siRNA 
      knockdown of SIRT1 mitigated the anti-oxidant and anti-inflammatory effects of CR 
      serum. CR exerts anti-oxidant and anti-inflammatory vascular effects, which are 
      likely mediated by circulating factors, in part, via a SIRT1-dependent pathway.
FAU - Csiszar, Anna
AU  - Csiszar A
AD  - Department of Physiology, New York Medical College, Valhalla, NY 10595, USA.
FAU - Labinskyy, Nazar
AU  - Labinskyy N
FAU - Jimenez, Rosario
AU  - Jimenez R
FAU - Pinto, John T
AU  - Pinto JT
FAU - Ballabh, Praveen
AU  - Ballabh P
FAU - Losonczy, Gyorgy
AU  - Losonczy G
FAU - Pearson, Kevin J
AU  - Pearson KJ
FAU - de Cabo, Rafael
AU  - de Cabo R
FAU - Ungvari, Zoltan
AU  - Ungvari Z
LA  - eng
GR  - R01 HL077256-02/HL/NHLBI NIH HHS/United States
GR  - R01 CA111842/CA/NCI NIH HHS/United States
GR  - R01 HL077256-03/HL/NHLBI NIH HHS/United States
GR  - HL077256/HL/NHLBI NIH HHS/United States
GR  - R01 HL077256-01A2/HL/NHLBI NIH HHS/United States
GR  - HL43023/HL/NHLBI NIH HHS/United States
GR  - R01 HL077256-04/HL/NHLBI NIH HHS/United States
GR  - R01 HL077256/HL/NHLBI NIH HHS/United States
GR  - CA111842/CA/NCI NIH HHS/United States
GR  - P01 HL043023/HL/NHLBI NIH HHS/United States
GR  - ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090621
PL  - Ireland
TA  - Mech Ageing Dev
JT  - Mechanisms of ageing and development
JID - 0347227
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (NF-kappa B)
RN  - 0 (Stilbenes)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - EC 3.5.1.- (Sirtuins)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - *Aging
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/*metabolism/pharmacology
MH  - *Caloric Restriction
MH  - Coronary Vessels/cytology
MH  - Endothelial Cells/cytology
MH  - Inflammation
MH  - Male
MH  - NF-kappa B/metabolism
MH  - Oxidative Stress
MH  - Rats
MH  - Rats, Inbred F344
MH  - Resveratrol
MH  - Sirtuin 1
MH  - Sirtuins/*physiology
MH  - Stilbenes/pharmacology
PMC - PMC2756526
MID - NIHMS138013
EDAT- 2009/06/25 09:00
MHDA- 2009/09/29 06:00
PMCR- 2010/08/01
CRDT- 2009/06/25 09:00
PHST- 2009/02/03 00:00 [received]
PHST- 2009/05/06 00:00 [revised]
PHST- 2009/06/15 00:00 [accepted]
PHST- 2009/06/25 09:00 [entrez]
PHST- 2009/06/25 09:00 [pubmed]
PHST- 2009/09/29 06:00 [medline]
PHST- 2010/08/01 00:00 [pmc-release]
AID - S0047-6374(09)00085-2 [pii]
AID - 10.1016/j.mad.2009.06.004 [doi]
PST - ppublish
SO  - Mech Ageing Dev. 2009 Aug;130(8):518-27. doi: 10.1016/j.mad.2009.06.004. Epub 
      2009 Jun 21.